Cell separation plays an important role in the fields of analytical chemistry and biomedicine. To solve the blockage problem and improve the separation throughput in the traditional microstructure filtration-based separation approach, a continuous cell separation and collection approach via micropost array railing on a microfilter and negative dielectrophoresis combined chip is proposed. By tilting the micropost array at a certain angle, microparticles or cells enter the collection area under micropost array railing. The effects of the inclination angle of the micropost array and the electrode distance on the microparticle collection efficiency were investigated. Based on the optimized microfluidic chip structure, 37- and 16.3-μm particles were collected with 85% and 89% efficiencies, respectively. Additionally, algal cells were separated and collected by using the optimized microchip. The chip also had good separation and collection effects on biological samples, which effectively solved the blockage problem and improved the separation throughput, laying a foundation for subsequent microstructure filtration separation-based research and application.

中文翻译：

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微滤与负介电电泳复合芯片的连续细胞分离与收集方法

细胞分离在分析化学和生物医学领域中起着重要作用。为了解决传统的基于微结构过滤的分离方法中存在的堵塞问题并提高分离效率，提出了一种通过微滤器上的微柱阵列栏杆和负介电电泳复合芯片的连续细胞分离和收集方法。通过将微柱阵列倾斜一定角度，微粒或细胞会在微柱阵列栏杆下进入收集区域。研究了微柱阵列的倾斜角度和电极距离对微粒收集效率的影响。基于优化的微流控芯片结构，分别收集了效率为85％和89％的37和16.3μm颗粒。另外，通过使用优化的微芯片分离并收集藻类细胞。该芯片对生物样品也具有良好的分离和收集效果，有效解决了堵塞问题，提高了分离通量，为后续基于微结构过滤分离的研究和应用奠定了基础。