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The Class III peroxidase gene OsPrx30, transcriptionally modulated by the AT‐hook protein OsATH1, mediates rice bacterial blight‐induced ROS accumulation
Journal of Integrative Plant Biology ( IF 9.3 ) Pub Date : 2020-11-26 , DOI: 10.1111/jipb.13040 Hao Liu 1, 2 , Shuangyu Dong 1 , Ming Li 1 , Fengwei Gu 1 , Guili Yang 1 , Tao Guo 1 , Zhiqiang Chen 1 , Jiafeng Wang 1
Journal of Integrative Plant Biology ( IF 9.3 ) Pub Date : 2020-11-26 , DOI: 10.1111/jipb.13040 Hao Liu 1, 2 , Shuangyu Dong 1 , Ming Li 1 , Fengwei Gu 1 , Guili Yang 1 , Tao Guo 1 , Zhiqiang Chen 1 , Jiafeng Wang 1
Affiliation
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Class III peroxidases (CIII Prxs) play critical roles in plant immunity by scavenging reactive oxygen species (ROS). However, the functions of CIII Prxs in rice (Oryza sativa L.) immunity are largely unexplored. Here, we report a Prx precursor, OsPrx30, that is responsive to the bacterial blight Xanthomonas oryzae pv. oryzae (Xoo). OsPrx30 was primarily expressed in rice roots, leaves, and stems, and its protein product was mainly localized at the endoplasmic reticulum. Overexpression of OsPrx30 enhanced the plant's susceptibility to Xoo by maintaining a high level of peroxidase (POD) activity and reducing the content of H2O2, whereas depletion of OsPrx30 had the opposite effects. Furthermore, we identified an AT‐hook transcription factor, OsATH1, that is specifically bound to the OsPrx30 promoter. As observed in plants overexpressing OsPrx30, depletion of OsATH1 enhanced susceptibility to Xoo. Finally, we demonstrated that depletion of OsATH1 increased histone H3 acetylation at the AT‐rich region of the OsPrx30 promoter. Taken together, these results reveal a mechanism underlying the POD‐induced natural resistance to bacterial diseases and suggest a model for transcription regulation of Prx genes in rice.
中文翻译：
由AT钩蛋白OsATH1转录调节的III类过氧化物酶基因OsPrx30介导水稻白叶枯病诱导的ROS积累
III类过氧化物酶（CIII Prxs）通过清除活性氧（ROS）在植物免疫中发挥关键作用。但是，CIII Prxs在水稻（Oryza sativa L.）免疫中的功能尚待开发。在这里，我们报告Prx前体OsPrx30，它对细菌性枯萎病Xanthomonas oryzae pv有反应。稻（Xoo）。OsPrx30主要在水稻的根，叶和茎中表达，其蛋白产物主要位于内质网。OsPrx30的过表达通过维持高水平的过氧化物酶（POD）活性和减少H 2 O含量而增强了植物对Xoo的敏感性。2，而消耗OsPrx30具有相反的效果。此外，我们鉴定了一个AT钩转录因子OsATH1，它与OsPrx30启动子特异性结合。如在过表达植株观察到OsPrx30，耗尽OsATH1增强易感性白叶枯病。最后，我们证明了OsATH1的耗竭增加了OsPrx30启动子的AT富集区的组蛋白H3乙酰化。综上所述，这些结果揭示了POD诱导的对细菌疾病的自然抗性的潜在机制，并提出了水稻Prx基因转录调控的模型。
更新日期：2020-11-26
中文翻译：
由AT钩蛋白OsATH1转录调节的III类过氧化物酶基因OsPrx30介导水稻白叶枯病诱导的ROS积累
III类过氧化物酶（CIII Prxs）通过清除活性氧（ROS）在植物免疫中发挥关键作用。但是，CIII Prxs在水稻（Oryza sativa L.）免疫中的功能尚待开发。在这里，我们报告Prx前体OsPrx30，它对细菌性枯萎病Xanthomonas oryzae pv有反应。稻（Xoo）。OsPrx30主要在水稻的根，叶和茎中表达，其蛋白产物主要位于内质网。OsPrx30的过表达通过维持高水平的过氧化物酶（POD）活性和减少H 2 O含量而增强了植物对Xoo的敏感性。2，而消耗OsPrx30具有相反的效果。此外，我们鉴定了一个AT钩转录因子OsATH1，它与OsPrx30启动子特异性结合。如在过表达植株观察到OsPrx30，耗尽OsATH1增强易感性白叶枯病。最后，我们证明了OsATH1的耗竭增加了OsPrx30启动子的AT富集区的组蛋白H3乙酰化。综上所述，这些结果揭示了POD诱导的对细菌疾病的自然抗性的潜在机制，并提出了水稻Prx基因转录调控的模型。
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