Trans‐epithelial electrical resistance (TEER) is a widely used non‐invasive and label‐free measurement to evaluate the barrier function of mono‐layered cells in real‐time. However, TEER values depend on distribution of electric current densities generated by a combination of shapes and arrangements of plane electrodes for performing TEER, but until now, there is no systematical analysis of their effects on TEER values, causing less reproducibility. Here, we report a new strategy to design the shapes and arrangements of electrodes to perform TEER in a microfluidic device. To investigate the effects of the electrodes design on TEER values, we analyze the distribution of electric current densities using finite element method and evaluate their uniformity of sensitivity. Our strategy allowed designing several electrode patterns of electrodes combinations, capable of simultaneous cell observation with a microscope and TEER measurements.

中文翻译：

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跨上皮电阻（TEER）测量的基于微流控设备中有限元分析的电极图案设计策略

跨上皮电阻（TEER）是一种广泛使用的无创且无标签的测量方法，可实时评估单层细胞的屏障功能。但是，TEER值取决于用于执行TEER的平面电极的形状和布置的组合所产生的电流密度的分布，但是直到现在，还没有系统地分析它们对TEER值的影响，从而导致再现性较低。在这里，我们报告了一种新的策略来设计电极的形状和布置，以在微流体设备中执行TEER。为了研究电极设计对TEER值的影响，我们使用有限元方法分析了电流密度的分布，并评估了其灵敏度的均匀性。