High‐Preservation Single‐Cell Operation through a Photo‐responsive Hydrogel‐Nanopipette System,Angewandte Chemie International Edition

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High‐Preservation Single‐Cell Operation through a Photo‐responsive Hydrogel‐Nanopipette System
Angewandte Chemie International Edition ( IF 16.1 ) Pub Date : 2020-11-26 , DOI: 10.1002/anie.202013011
Zi-Yuan Li ₁ , Ying-Ya Liu ₁ , Yuan-Jie Li ₁ , Wenhui Wang ₁ , Yanyan Song ₁ , Junji Zhang ₁ , He Tian ₁

Affiliation

Single‐cell and in situ cell‐based operation with nanopipette approach offers a possibility to elucidate the intracellular processes and may aid the improvement of therapy efficiency and precision. We present here a photo‐responsive hydrogel‐nanopipette hybrid system that can achieve single‐cell operation with high spatial/temporal resolution and negligible cell damage. This strategy overcomes long‐time obstacles in nanopipette single‐cell studies as high electric potential (ca. 1000 mV) or organic solvent is always used during operations, which would inevitably impose disturbance and damage to targeted cells. The light‐triggered system promotes a potential‐free, non‐invasive single‐cell injection, resulting in a well‐retained cell viability (90 % survival rate). Moreover, the photo‐driven injection enables a precisely dose‐controllable single‐cell drug delivery. Significantly reduced lethal doses of doxorubicin (163–217 fg cell⁻¹) are demonstrated in corresponding cell lines.

中文翻译：

通过光响应水凝胶纳米移液器系统实现高保存度的单细胞操作

采用纳米移液管方法的单细胞和原位细胞操作为阐明细胞内过程提供了可能，并可能有助于提高治疗效率和精度。我们在这里介绍一种光响应水凝胶-纳米移液器混合系统，该系统可以实现具有高时空分辨率和可忽略的细胞损伤的单细胞操作。该策略克服了纳米移液器单细胞研究中的长期障碍，因为在操作过程中始终使用高电势（约1000 mV）或有机溶剂，这势必会对目标细胞造成干扰和损害。轻触发的系统可促进无电势，非侵入性的单细胞注射，从而使细胞存活率保持良好（存活率90％）。而且，光驱动注射可实现精确剂量可控的单细胞药物输送。显着降低了阿霉素的致死剂量（163–217 fg细胞^-1）在相应的细胞系中得到证实。

更新日期：2020-11-26

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