There is an urgent need to find efficient methods to remediate polluted manure by ionophore antibiotics (IPAs). In the present study, we tried a novel bioremediation strategy for monensin (MON, one of IPAs) contaminated chicken manure through a combined bioaugmentation-vermicomposting approach with housefly larvae and MON-degrading bacteria. Under aerobic conditions, 31.37% of MON was degraded over 12 days through bioaugmentation using only Stenotrophomonas sp. DM-2, while the degradation rate of MON was 88.15% under the combined action of the larvae and Stenotrophomonas sp. DM-2. It was found that housefly larvae activity could change the pH and temperature of chicken manure and then reshaped the microbial community structure of chicken manure substrates. This activity of larvae enhanced the abundance of MON-degrading bacterial genera (Stenotrophomonas and Alcaligenes), making the genera Pseudogracilibacillus, Pseudomonas, Oligella, and Stenotrophomonas dominant after a 12-day bioremediation period, which contributed to the marked reduction of MON in manure. A novel potential degradation pathway of MON in chicken manure was also speculated based on the identification and characterization from LC-QToF/MS spectral data of two new MON metabolic intermediates. The present study provided a novel efficient method to remediate IPAs contaminated manure and provide new insights into the microbial molecular ecological mechanisms for bioremediation of MON contaminated chicken manure.

迫切需要找到有效的方法来修复离子载体抗生素（IPA）污染的粪便。在本研究中，我们通过结合家蝇幼虫和MON降解细菌的生物强化-防堆肥方法，尝试了一种针对莫能菌素（MON，IPA之一）污染的鸡粪的新型生物修复策略。在有氧条件下，仅使用嗜麦芽单胞菌属物种，通过生物增强作用，在12天内降解了31.37％的MON 。DM-2，而在幼虫和嗜麦芽窄食单胞菌的共同作用下，MON的降解率为88.15％sp。DM-2。研究发现，家蝇幼虫的活性可以改变鸡粪的pH值和温度，进而改变鸡粪基质的微生物群落结构。幼虫的这种活性增强了MON降解细菌属（嗜麦芽单胞菌和产碱菌）的丰富度，使假单胞菌属，假单胞菌属，寡果类和嗜麦芽单胞菌属经过12天的生物修复期后成为显性细菌，这显着降低了粪便中MON含量。根据两种新的MON代谢中间体的LC-QToF / MS光谱数据的鉴定和表征，还推测了MON在鸡粪中的潜在降解途径。本研究提供了一种新的有效方法来修复受IPA污染的粪便，并为微生物修复MON污染的鸡粪的微生物分子生态机制提供了新的见解。