Peroxynitrite (ONOO⁻), a potent oxidative and nitrating reagent that can damage a variety of molecules in cells is reported to be closely related to various diseases. To determine the physiological or pathophysiological concentrations of ONOO⁻ and to deeply understand its pathophysiological processes strongly rely on the development of more powerful detection methods. Hereof, we fabricated the first ratiometric fluorescent probe by using aggregation-induced emission luminogens (AIEgens) with superior photostability for efficient and reliable visualization of ONOO⁻ both in vitro and in vivo. Probe BTCV-PN, obtained via simple two-step process, displayed superior photostability, remarkable sensitivity and outstanding specificity toward ONOO⁻ through a ratiometric response mode. Furthermore, BTCV-PN was successfully applied for imaging of exogenous and endogenous ONOO⁻ in live cells. Notably, in vivo imaging of ONOO⁻ in live mice was also accomplished. The excellent performances plus the superior parameters of BTCV-PN including reliable ratiometric response and superior stability against photobleaching would not only make BTCV-PN a powerful tool-box for facilely investigating the cellular concentrations of ONOO⁻, but also open up an updated way for straightforward visualization of the pathophysiological process of ONOO⁻ in live cells and animals. Elsevier B.V. All rights reserved.

过氧亚硝酸盐（ONOO ^- ），一种有效的氧化和硝化试剂，可以破坏的各种细胞分子被报告给密切相关的各种疾病。为了确定ONOO的生理或病理生理浓度^-并深入了解其病理生理过程强烈依赖于更强大的检测方法的发展。书中，我们通过使用具有用于ONOO的有效和可靠的可视化优良的耐光性聚合诱导的发射luminogens（AIEgens）制造的第一比率荧光探针^-既在体外和体内。探针BTCV-PN，获得通过简单的两步过程，显示优异的耐光性，显着的灵敏度和特异性优秀朝向ONOO ^-通过比例响应模式。此外，BTCV-PN被成功地应用于外源性和内源性ONOO的成像^-在活细胞中。值得注意的是，在体内ONOO成像^-活体小鼠也已实现。出色的表演加上BTCV-PN优越的参数，包括可靠的比率响应和卓越的稳定性，对漂白将不仅使BTCV-PN的有力工具盒的轻便调查ONOO的细胞浓度^{- ，}而且开拓了一个更新的方式直接观察ONOO的病理生理过程⁻在活细胞和动物中。Elsevier BV保留所有权利。