Celtica gigantea(= Stipa gigantea) is a large perennial grass which grows in nutrient-poor sandy soils in semiarid zones of the western Iberian Peninsula. The purpose of this work was to find out if culturable fungal symbionts isolated from roots of this wild grass could have growth promoting activity in tritordeum, a hybrid cereal for human consumption. A survey of fungi from the root endosphere of C. gigantea produced an isolate collection consisting of 60 different taxa, mostly ascomycetes. Fungal strains were inoculated into tritordeum plants in order to evaluate their effect in leaf and root biomass, nutrient content, and total antioxidant capacity. Two consecutive screening processes were made to test endophyte effects in plants. In the first screening, 66 strains were inoculated into seedlings by dipping roots in a liquid suspension of inoculum. In the second screening, 13 strains selected from the first screening were inoculated by sowing seeds in a substrate containing inoculum. The inoculation method used in the second screening involved less labor and plant manipulation and improved the quantity and quality of the inoculum, making it more appropriate for big scale experimental inoculation procedures. Several fungal strains promoted leaf or root growth. In particular, a strain belonging to the genus Diaporthe caused an increase in leaf and root biomass in both screening processes, suggesting that this endophyte might have a good potential for field application in tritordeum.

中文翻译：

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从野草中筛选真菌内生菌以促进农业谷物 tritordeum 的生长

Celtica gigantea（= Stipa gigantea）是一种大型多年生草，生长在伊比利亚半岛西部半干旱地区营养贫乏的沙质土壤中。这项工作的目的是找出从这种野草根部分离的可培养真菌共生体是否可以在 tritordeum（一种供人类食用的杂交谷物）中具有促进生长的活性。对 C. gigantea 根内层真菌的调查产生了一个由 60 个不同分类群组成的分离集合，主要是子囊菌。将真菌菌株接种到 tritordeum 植物中，以评估它们对叶和根生物量、营养成分和总抗氧化能力的影响。进行了两个连续的筛选过程以测试植物中的内生菌效应。在第一场放映中，通过将根浸入接种物的液体悬浮液中，将 66 个菌株接种到幼苗中。在第二次筛选中，从第一次筛选中选出的 13 个菌株通过将种子播种在含有接种物的基质中进行接种。第二次筛选采用的接种方法，劳动和植物操作少，提高了接种物的数量和质量，使其更适合大规模实验接种程序。几种真菌菌株促进了叶或根的生长。特别是，一种属于 Diaporthe 属的菌株在两种筛选过程中都引起了叶和根生物量的增加，这表明这种内生菌可能在 tritordeum 中具有良好的田间应用潜力。通过将种子播种在含有接种物的基质中，从第一次筛选中选出的 13 个菌株进行接种。第二次筛选采用的接种方法，劳动和植物操作少，提高了接种物的数量和质量，使其更适合大规模实验接种程序。几种真菌菌株促进了叶或根的生长。特别是，属于 Diaporthe 属的菌株在两种筛选过程中都引起了叶和根生物量的增加，这表明这种内生菌可能在 tritordeum 中具有良好的田间应用潜力。通过将种子播种在含有接种物的基质中，从第一次筛选中选出的 13 个菌株进行接种。第二次筛选采用的接种方法，劳动和植物操作少，提高了接种物的数量和质量，使其更适合大规模实验接种程序。几种真菌菌株促进了叶或根的生长。特别是，属于 Diaporthe 属的菌株在两种筛选过程中都引起了叶和根生物量的增加，这表明这种内生菌可能在 tritordeum 中具有良好的田间应用潜力。使其更适合大规模实验接种程序。几种真菌菌株促进了叶或根的生长。特别是，属于 Diaporthe 属的菌株在两种筛选过程中都引起了叶和根生物量的增加，这表明这种内生菌可能在 tritordeum 中具有良好的田间应用潜力。使其更适合大规模实验接种程序。几种真菌菌株促进了叶或根的生长。特别是，属于 Diaporthe 属的菌株在两种筛选过程中都引起了叶和根生物量的增加，这表明这种内生菌可能在 tritordeum 中具有良好的田间应用潜力。