The present study aimed to evaluate the oral ulcer healing process treated with Marine Collagen Peptides (MCPs) from tilapia skin. Oral ulcer models were established on the tongue mucosa of C57/BL6 mice by phenol liquids. 60 mice were randomly divided into four groups: blank group, normal saline group (NS), low concentration MCPs group (LMCPs) and high concentration MCPs group (HMCPs). Each substance was respectively administered on the ulcer area once daily and continued until sacrificed (after 1, 3, 5, 7 and 9 days of ulcer establishment). In addition to clinical and macroscopic evaluation, we also performed histological, histomorphometric and immunohistochemistry analysis. The results indicated that MCPs could accelerate the oral ulcer healing process effectively as a result of the high content Pro-Hyp effects. Firstly it reduced the numbers of inflammatory cells infiltration. Secondly it promoted collagenesis, angiogenesis and re-epithelialization from D5. Thirdly, it down-regulate the expressions of IL-1β and TNF-α in the mid-late stage. In general, MCPs has great potential for developing clinical applications on oral ulcer treatments.

本研究旨在评估用罗非鱼皮肤海洋胶原蛋白肽（MCP）处理的口腔溃疡愈合过程。用酚液在C57 / BL6小鼠舌黏膜上建立口腔溃疡模型。60只小鼠随机分为四组：空白组，生理盐水组（NS），低浓度MCP组（LMCP）和高浓度MCP组（HMCP）。每天分别在溃疡区域施用每种物质一次，并持续直至处死（在溃疡形成1、3、5、7和9天后）。除临床和宏观评估外，我们还进行了组织学，组织形态学和免疫组化分析。结果表明，由于高含量的Pro-Hyp效应，MCP可以有效地促进口腔溃疡的愈合过程。首先，它减少了炎症细胞浸润的次数。其次，它促进了D5的胶原蛋白形成，血管生成和再上皮形成。第三，它在中晚期下调IL-1β和TNF-α的表达。通常，MCP具有开发口腔溃疡治疗临床应用的巨大潜力。