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Construction of simple, customised, brain-spanning, multi-channel, linear microelectrode arrays
Journal of Neuroscience Methods ( IF 2.7 ) Pub Date : 2020-11-27 , DOI: 10.1016/j.jneumeth.2020.109011
Ashik Banstola 1 , Carlos Silva 1 , Katharina Ulrich 1 , Ming Ruan 1 , Lindsay Robertson 1 , Neil McNaughton 1
Affiliation  

Background

The construction of multichannel micro-electrode arrays (MEA) generally requires complex and expensive procedures. Here we describe a simple, cheap, flexible method of linear MEA construction.

New method

Our method allows manufacture of linear MEA (cross section ∼ 375 × 250 μm with 12 electrodes) of any desired length, with customized spacing of the electrode tips (down to a minimum spacing of 200 μm or less) to suit different needs and experiments. We describe the following steps: (1) set-up for MEA construction; (2) building of a construction jig; (3) building the reference, ground and optional electrical stimulation electrodes; (4) treatment of the main recording microwires; (5) soldering of the microwires to the main connector plug and arrangement of the microwires in a customizable array; and (6) testing of the MEA resistance and correct connections. Finally, we describe methods for quick surgical implantation of multiple MEAs and bipolar micro-stimulation electrodes for in vivo experiments in free-moving rats.

Results

We provide examples of multi-site local field potentials from prolonged recordings in awake and free-moving rodents, with recordings viable for months, as well as samples of electrical stimulation effects on cortical and hippocampal recordings. Hippocampal recordings showed clear phase reversal and amplitude changes across its layers.

Conclusions

We briefly discuss how the arrays can support other forms of stimulation such as optogenetic probes.



中文翻译:

构建简单,定制的跨脑多通道线性微电极阵列

背景

多通道微电极阵列(MEA)的构造通常需要复杂且昂贵的过程。在这里,我们描述了一种简单,便宜,灵活的线性MEA构建方法。

新方法

我们的方法允许制造任何所需长度的线性MEA(横截面〜375 x 250 µm,带12个电极),并定制化的电极头间距(最小间距为200 µm或更小),以适应不同的需求和实验。我们描述了以下步骤:(1)建立MEA结构;(二)建造施工夹具;(3)建立参考,接地和可选的电刺激电极;(4)主记录微线的处理;(5)将微线焊接到主连接器插头上,并将微线布置成可定制的阵列;(6)测试MEA电阻和正确的连接。最后,我们描述了用于体内多个MEA和双极微刺激电极的快速外科植入方法 自由移动大鼠的实验。

结果

我们提供了在清醒和自由移动的啮齿动物中长时间录音的多站点局部电场的示例,这些录音可以持续几个月的时间,以及对皮质和海马录音的电刺激效果的样本。海马的记录显示出清晰的相位反转和幅度变化。

结论

我们简要讨论了阵列如何支持其他形式的刺激,例如光遗传学探针。

更新日期:2020-12-05
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