A simple and effective analytical method to determine six contaminants, including five benzophenones (BP, BP-1, BP-3, BP-8, and BP-12) and bisphenol A (BPA) in infant formulas was developed in this study. For this, a sequential experimental design was used to optimize the extraction and cleanup method using low temperature partition (LTP) combined with dispersive solid phase extraction (dSPE). The effect of primary secondary amine (PSA), sodium chloride (NaCl), graphitized carbon black (GCB), octadecyl (C18), strong anion exchanger (SAX), water, acetonitrile (ACN) and, ultrasound (US) time were evaluated using a sequential design of experiments including a Plackett-Burman, a central composite rotatable design, and the Derringer and Suich's tool. The method was validated, and it showed a limit of quantification varying from 0.06 to 2 mg.kg⁻¹, good precision (< 20% RSD), and recovery (52-106%). The method proposed was applied to twenty-five samples of commercial infant formulas.

本研究开发了一种简单有效的分析方法，可测定婴儿配方食品中的六种污染物，包括五种二苯甲酮（BP，BP-1，BP-3，BP-8和BP-12）和双酚A（BPA）。为此，使用了顺序实验设计来优化提取和净化方法，该方法使用了低温分配（LTP）和分散固相提取（dSPE）。评估了伯仲胺（PSA），氯化钠（NaCl），石墨化炭黑（GCB），十八烷基（C18），强阴离子交换剂（SAX），水，乙腈（ACN）和超声（US）时间的影响使用了包括Plackett-Burman，中央复合材料可旋转设计以及Derringer和Suich的工具的顺序实验设计。该方法经过验证，显示的定量下限为0.06至2 mg.kg^-1，良好的精度（<20％RSD）和恢复（52-106％）。提议的方法已应用于25个商业婴儿配方奶粉样品。