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Production of medium-chain-length polyhydroxyalkanoates by Pseudomonas chlororaphis subsp. aurantiaca: Cultivation on fruit pulp waste and polymer characterization
International Journal of Biological Macromolecules ( IF 7.7 ) Pub Date : 2020-11-27 , DOI: 10.1016/j.ijbiomac.2020.11.162
João R. Pereira , Diana Araújo , Patrícia Freitas , Ana C. Marques , Vítor D. Alves , Chantal Sevrin , Christian Grandfils , Elvira Fortunato , Maria A.M. Reis , Filomena Freitas

Pseudomonas chlororaphis subsp. aurantiaca DSM 19603 was cultivated on apple pulp, a glucose- and fructose-rich waste generated during juice production, to produce medium-chain length polyhydroxyalkanoates. A cell dry mass of 8.74 ± 0.20 g/L, with a polymer content of 49.25 ± 4.08% were attained. The produced biopolymer was composed of 42.7 ± 0.1 mol% 3-hydroxydecanoate, 17.9 ± 1.0 mol% 3-hydroxyoctanoate, 14.5 ± 1.1 mol% 3-hydroxybutyrate, 11.1 ± 0.6 mol% 3-hydroxytetradecanoate, 10.1 ± 0.5 mol% 3-hydroxydodecanoate and 3.7 ± 0.2 mol% 3-hydroxyhexanoate. It presented low glass transition and melting temperatures (−40.9 ± 0.7 °C and 42.0 ± 0.1 °C, respectively), and a degradation temperature of 300.0 ± 0.1 °C, coupled to a low crystallinity index (12.7 ± 2.7%), a molecular weight (Mw) of 1.34 × 105 ± 0.18 × 105 Da and a polydispersity index of 2.70 ± 0.03. The biopolymer's films were dense and had a smooth surface, as demonstrated by Scanning Electron Microscopy. They presented a tension at break of 5.21 ± 1.09 MPa, together with an elongation of 400.5 ± 55.8% and an associated Young modulus of 4.86 ± 1.49 MPa, under tensile tests. These attractive filming properties of this biopolymer could potentially be valorised in several areas such as the fine chemicals industry, biomedicine, pharmaceuticals, or food packaging.



中文翻译:

绿假单胞菌亚种生产中链长度的多羟基链烷酸酯。aurantiaca:果肉废料的培养和聚合物的表征

绿假单胞菌亚种。将aurantiaca DSM 19603种植在苹果浆上,这是在果汁生产过程中产生的富含葡萄糖和果糖的废物,可生产中链长度的聚羟基链烷酸酯。获得了8.74±0.20g / L的细胞干质量,聚合物含量为49.25±4.08%。产生的生物聚合物由42.7±0.1 mol%3-羟基癸酸酯,17.9±1.0 mol%3-羟基辛酸酯,14.5±1.1 mol%3-羟基丁酸酯,11.1±0.6 mol%3-羟基十四酸酯,10.1±0.5 mol%3-羟基十二酸酯组成和3.7±0.2mol%的3-羟基己酸酯。它具有较低的玻璃化转变温度和熔融温度(分别为−40.9±0.7°C和42.0±0.1°C)和300.0±0.1°C的降解温度,以及较低的结晶度指数(12.7±2.7%),分子量(Mw)1.34×105  ±0.18×10 5  Da,多分散指数为2.70±0.03。如扫描电子显微镜所证明的,生物聚合物的膜致密且具有光滑的表面。在拉伸试验中,他们表现出的断裂张力为5.21±1.09 MPa,伸长率为400.5±55.8%,相关的杨氏模量为4.86±1.49 MPa。这种生物聚合物的这些吸引人的成膜特性可能会在诸如精细化工,生物医学,制药或食品包装等多个领域得到重视。

更新日期:2020-12-02
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