Antibacterial wound dressings can effectively avoid the residual of antibacterial nanomaterials for injection in vivo, reduce their biological toxicity to normal cells and tissues, making them be widely applied in biomedical field. Herein, an approach of combining ion-crosslinking, in-situ reduction and microwave-assisted methods was employed to prepare chitosan-copper-gallic acid nanocomposites (CS-Cu-GA NCs) with dual-functional nano-enzyme characteristics (oxidase- and peroxidase-like functions). The oxidase-like activity of CS-Cu-GA NCs can facilitate the production of hydrogen peroxide (H₂O₂) when it contacted with physiologically relevant antioxidants (AH₂) in bacteria. Subsequently, H₂O₂ was catalyzed to generate hydroxyl radicals (OH) under the peroxidase-like activity of CS-Cu-GA NCs. Furthermore, CS-Cu-GA NCs integrate the inherent antibacterial properties of chitosan, Cu NPs and Cu²⁺. Animal experiments revealed that the antibacterial dressing incorporating CS-Cu-GA NCs exhibited its effective promotion of S. aureus-infected wounds healing, as well as no damage to normal tissues. Besides, the antibacterial dressing was prepared to a band aid with excellent water swelling and antibacterial properties, which was further fixed in a medical tape to construct a portable antibacterial product that can be applied to the surface of human skin and showed excellent waterproof performance, providing a new insight for the construction of clinical antibacterial wound healing products.

抗菌伤口敷料可有效避免体内注射用抗菌纳米材料的残留，降低其对正常细胞和组织的生物毒性，使其在生物医学领域得到广泛应用。本文采用离子交联，原位还原和微波辅助相结合的方法制备具有双重功能的纳米酶特性（氧化酶和氧化酶）的壳聚糖-铜-没食子酸纳米复合材料（CS-Cu-GA NCs）。过氧化物酶样功能）。当CS-Cu-GA NCs与细菌中与生理相关的抗氧化剂（AH ₂）接触时，其类似于氧化酶的活性可促进过氧化氢（H ₂ O ₂）的产生。随后，H ₂在CS-Cu-GA NCs的过氧化物酶样活性下，O ₂被催化生成羟基自由基（OH）。此外，CS-Cu-GA NCs还具有壳聚糖，Cu NPs和Cu ²⁺固有的抗菌特性。动物实验表明，结合CS-Cu-GA NCs的抗菌敷料具有有效促进金黄色葡萄球菌的作用。-感染的伤口愈合，并且对正常组织没有损害。此外，将抗菌敷料制备为具有优异的水溶胀和抗菌性能的创可贴，将其进一步固定在医用胶带上，制成一种可应用于人体皮肤表面并显示出优异防水性能的便携式抗菌产品，提供为临床抗菌伤口愈合产品的构建提供了新见解。