Statistical optimization of hydrolytic conditions for the production of fructooligosaccharides (FOSs) from pure inulin using Aspergillus tritici endoinulinase was carried out in a batch system. FOSs yield 99.19% was obtained under the optimized hydrolytic conditions i.e. inulin concentration (7.3%), enzyme load (65 IU), hydrolysis time (13h) and agitation (100 rpm). The closeness of value of co-efficient of determination (R²) to 1, good agreement between model's predicted and experimental values, low percentage error (<5%), high adequate precision (˃4%) and F value (11,634.32), and low Lack of fit (0.60) of the designed model authenticates its fitness. High substrate concentration, low enzyme load and short hydrolysis span justifies efficiency of developed process for the preparation of FOSs from inulin using fungal endoinulinase. TLC chromatographic and densitometry studies confirmed the synthesis of short-chain length FOSs. FOSs preparation contained 33.85% GF2 (ketose), 24.50% GF3 (nystose), 7.26% GF4 (fructofuranosylnystose) and 33.58% FOSs of DP5–9.

在分批系统中进行了使用小麦曲霉内切菊糖酶从纯菊粉生产果糖低聚糖（FOS）的水解条件的统计优化。在最佳水解条件下，即菊粉浓度（7.3％），酶负荷（65 IU），水解时间（13 h）和搅拌（100 rpm），FOS的收率达99.19％。测定系数（R ²）的值接近于1，模型的预测值与实验值之间具有良好的一致性，较低的百分比误差（<5％），较高的适当精度（˃4％）和F值（11,634.32），且设计模型的拟合度低（0.60）低，验证了其适用性。高底物浓度，低酶负荷和短水解时间证明了使用真菌内切菊糖酶从菊粉制备FOS的开发方法的效率是合理的。TLC色谱和光密度法研究证实了短链长度FOS的合成。FOSs制剂包含33.85％的GF2（酮糖），24.50％的GF3（果糖），7.26％的GF4（果糖呋喃糖基nystose）和33.58％的DP5-9的FOS。