Purpose

To evaluate cytokine production in vitro by different types of leukocytes stimulated with monosodium urate (MSU), calcium pyrophosphate (CPP) and basic calcium phosphate (BCP) crystals.

Material and methods

Polymorphonuclear cells (PMN), monocytes and lymphocytes, isolated from healthy volunteer blood, were stimulated for different time periods with increasing MSU, CPP or BCP crystal concentrations. IL-1β, IL-8, IL-6, CCL2, IL-1Ra and TGFβ1 were determined by ELISA.

Results

Exposure of PMN to different crystals resulted in a moderate IL-8 and IL-1Ra release. Stimulation of monocytes induced a significant production of all the cytokines evaluated. The highest levels of IL-1β, IL-6, CCL2 and IL-8 were observed with MSU at 0.5 mg/ml, CPP at 0.01–0.05 mg/ml and BCP at 1 mg/ml after 18–48 h and then decreased. At the same crystal concentrations, IL-1Ra and TGFβ1 increased until the end of the experiment. Treatment of lymphocytes with different crystals did not induce cytokine release.

Conclusion

This study demonstrates that PMN, monocytes and lymphocytes from the same donor respond differently after stimulation with MSU, CPP or BCP crystals, depending on the dose and the time of exposure. Crystals induce a rapid increase of pro-inflammatory cytokines, whereas longer time is required to release high levels of anti-inflammatory cytokines.

中文翻译：

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致病晶体对不同白细胞群体产生促炎和抗炎细胞因子的影响

目的

评估用尿酸单钠 (MSU)、焦磷酸钙 (CPP) 和碱性磷酸钙 (BCP) 晶体刺激的不同类型白细胞在体外产生的细胞因子。

材料与方法

随着 MSU、CPP 或 BCP 晶体浓度的增加，从健康志愿者血液中分离出的多形核细胞 (PMN)、单核细胞和淋巴细胞受到不同时间段的刺激。通过ELISA测定IL-1β、IL-8、IL-6、CCL2、IL-1Ra和TGFβ1。

结果

PMN 暴露于不同的晶体导致适度的 IL-8 和 IL-1Ra 释放。单核细胞的刺激诱导了所有评估的细胞因子的显着产生。在 0.5 mg/ml 的 MSU、0.01-0.05 mg/ml 的 CPP 和 1 mg/ml 的 BCP 在 18-48 小时后观察到最高水平的 IL-1β、IL-6、CCL2 和 IL-8，然后下降. 在相同的晶体浓度下，IL-1Ra 和 TGFβ1 增加，直到实验结束。用不同晶体处理淋巴细胞不会诱导细胞因子释放。

结论

该研究表明，来自同一供体的 PMN、单核细胞和淋巴细胞在 MSU、CPP 或 BCP 晶体刺激后反应不同，具体取决于暴露的剂量和时间。晶体诱导促炎细胞因子的快速增加，而需要更长的时间来释放高水平的抗炎细胞因子。