Listeria monocytogenes can form persistent biofilms on food processing surfaces, resulting in cross-contamination of food products, including milk and milk products. Natural glycolipids are a promising intervention to control undesirable microbes due to their antimicrobial activity and low toxicity. This study aimed to determine the antimicrobial activity of glycolipids to control L. monocytogenes biofilms as well as in milk and on Queso Fresco. Application of a natural glycolipid product significantly reduced biofilm-associated L. monocytogenes on both polystyrene and stainless steel at concentrations as low as 45 mg/L. When added to UHT skim milk, a concentration of 1000 mg/L inhibited L. monocytogenes growth through 7 days of storage at 7 °C, and application of 1300 and 1500 mg/L reduced counts to levels below the limit of enumeration at day 21. In contrast, 2000 mg/L were necessary to inhibit growth through 7 days in whole milk. Glycolipid solutions at concentrations ≥10% reduced L. monocytogenes counts on Queso Fresco through 7 days when applied as a dip. Overall, natural glycolipids have potential as a natural alternative for the removal of biofilms and as an antimicrobial to control L. monocytogenes in milk and milk products with short shelf lives.

单核细胞增生李斯特菌可在食品加工表面上形成持久的生物膜，从而导致食品的交叉污染，包括牛奶和奶制品。天然糖脂由于其抗菌活性和低毒性，是控制不良微生物的一种有前途的干预措施。这项研究旨在确定糖脂对控制单核细胞增生李斯特菌生物膜以及在牛奶中和在Queso Fresco上的抗菌活性。天然糖脂产品的应用可在低至45 mg / L的浓度下显着减少聚苯乙烯和不锈钢上与生物膜相关的单核细胞增生李斯特氏菌。当添加到UHT脱脂牛奶中时，浓度为1000 mg / L会抑制单核细胞增生李斯特菌通过在7°C下储存7天实现生长，并且在第21天施用1300和1500 mg / L可以将计数降低到低于计数上限的水平。相反，需要2000 mg / L来抑制整个7天的生长牛奶。浓度为≥10％的糖脂溶液在浸泡7天后，在Queso Fresco上减少了单核细胞增生李斯特菌计数。总体而言，天然糖脂具有作为去除生物膜的天然替代品以及作为控制货架期短的牛奶和奶制品中单核细胞增生李斯特氏菌的抗菌剂的潜力。