Abstract Symphytum species, commonly known as comfrey, hold a long history of folk medicinal uses for healing wounds, bone fractures and other pain resulting from inflammation. However, many species belonging to this genus have not yet been scientifically investigated and hence investigation in this regard is warranted. In the present research, S. aintabicum extracts prepared by different extraction techniques (homogenizer assisted extraction, infusion and maceration) were assayed for their chemosystematic attributes (total phenolic, flavonoid) as well as bioactive compound contents, antioxidant and enzyme inhibition potentials. For instance, the extracts were found to possess phenolic and flavonoid levels ranging from 35.50 to 112.25 mg GAE/g and 2.54–25.12 mg RE/g, respectively. In general, the extracts displayed significant antioxidant activity. However, methanolic and infusion extracts were observed as the most potent radical scavengers and reducing agents, most likely related to their phenolic content. The extracts also showed metal chelating power and total antioxidant capacity in phosphomolybdenum assay. Moreover, while all extracts showed inhibition on amylase, only the methanolic extracts acted as inhibitors of cholinesterases and tyrosinase, most probably due to their relatively higher flavonoid content. The findings also indicated that the extraction method as well as the solvent type used to more or less affect the yield of the bioactive compounds and extracts’ bioactivity reported herein. Hence, as a first report highlighting the in vitro pharmacological properties of S. aintabicum, promising results were revealed from the quantitative chemosystematic analysis of bioactive components present and as sources of antioxidants and enzyme inhibitors against key human illnesses, thus providing scope for potential applications in phytomedicine development.

中文翻译：

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Symphytum aintabicum Hub.-Mor. 不同提取物的化学成分和生物活性的初步研究。威肯斯(&Wickens)

摘要 Symphytum 物种，俗称紫草，拥有悠久的民间药用历史，可用于治疗伤口、骨折和其他炎症引起的疼痛。然而，许多属于该属的物种尚未经过科学调查，因此有必要进行这方面的调查。在本研究中，分析了通过不同提取技术（均质机辅助提取、浸渍和浸渍）制备的 S. aintabicum 提取物的化学系统属性（总酚类、类黄酮）以及生物活性化合物含量、抗氧化剂和酶抑制潜力。例如，发现提取物的酚类和类黄酮含量范围分别为 35.50 至 112.25 mg GAE/g 和 2.54-25.12 mg RE/g。一般来说，提取物显示出显着的抗氧化活性。然而，甲醇提取物和浸渍提取物被认为是最有效的自由基清除剂和还原剂，这很可能与其酚类含量有关。在磷钼测定中，提取物还显示出金属螯合能力和总抗氧化能力。此外，虽然所有提取物都显示出对淀粉酶的抑制作用，但只有甲醇提取物可作为胆碱酯酶和酪氨酸酶的抑制剂，这很可能是因为它们的类黄酮含量相对较高。研究结果还表明，提取方法以及所使用的溶剂类型或多或少会影响本文报道的生物活性化合物的产量和提取物的生物活性。因此，作为第一份强调 S. aintabicum 体外药理特性的报告，