The purpose of this study was to develop a rapid and simple measurement method for biofilm formation inhibitory activity, and to screen food additives and foodstuffs that inhibit biofilm formation. The measurement method for biofilm formation inhibitory activity was developed by combining biofilm formation on pins of microtiter plate lids and staining using crystal violet. The optimum conditions for biofilm formation on the pins were established for seven Gram-positive and six Gram-negative bacteria by investigations of media, incubation time, and pin materials. Minimum concentrations of food additives required to inhibit biofilm formation were determined using the proposed method. The values obtained by the proposed and conventional methods agreed well. In addition, by sequential measurements of minimum inhibitory concentrations and minimum bactericidal concentrations using the proposed method, mechanisms of inhibition of biofilm formation were assessed. Furthermore, inhibitory activities of the water extracts of 498 different plant foodstuffs on biofilm formation by Streptococcus mutans were measured; five of the extracts showed potent inhibitory activities. The method proposed here circumvents the tedious and time-consuming conventional method in which biofilms are cultivated on the bottom of wells of microtiter plates.

中文翻译：

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使用 96 针微量滴定板盖快速简单地测量生物膜形成抑制活性的方法

本研究的目的是开发一种快速简单的生物膜形成抑制活性测量方法，并筛选抑制生物膜形成的食品添加剂和食品。生物膜形成抑制活性的测量方法是通过将微量滴定板盖针上的生物膜形成和使用结晶紫染色相结合而开发的。通过对培养基、孵育时间和针材料的研究，为 7 种革兰氏阳性菌和 6 种革兰氏阴性菌确定了针上生物膜形成的最佳条件。使用建议的方法确定抑制生物膜形成所需的食品添加剂的最低浓度。通过提出的方法和常规方法获得的值非常吻合。此外，通过使用所提出的方法连续测量最低抑菌浓度和最低杀菌浓度，评估了抑制生物膜形成的机制。此外，还测量了 498 种不同植物食品的水提取物对变形链球菌生物膜形成的抑制活性；五种提取物显示出有效的抑制活性。这里提出的方法避免了在微量滴定板孔底培养生物膜的繁琐和耗时的传统方法。五种提取物显示出有效的抑制活性。这里提出的方法避免了在微量滴定板孔底培养生物膜的繁琐和耗时的传统方法。五种提取物显示出有效的抑制活性。这里提出的方法避免了在微量滴定板孔底培养生物膜的繁琐和耗时的传统方法。