Prostate cancer is a leading health burden, the third most common cancer in a man. High accuracy detection and screening methods with a suitable biomarker can significantly reduce the risk of mortality. Prostate specific antigen (PSA) is the efficient and acceptable biomarker due to its level of increment in the biological fluid with the prostate cancer patient. This research was focused to establish a sensitive method of PSA detection by using gold nanoparticle (GNP) conjugated PSA specific aptamer on interdigitated mini-electrode. GNP allowed to capture higher number of aptamers on the surface and enhanced the interaction of PSA. This good detection method can determine PSA at 45[Formula: see text]aM with the sensitivity of 30[Formula: see text]aM. A linear range was noticed from 60 until 2000 aM on the regression curve at [Formula: see text]; [Formula: see text]. Moreover, spiking PSA in human serum enhances the current response with increasing PSA concentrations. This method of determination helps to quantify the PSA level and diagnose the prostate cancer at different stages.

中文翻译：

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聚乙二醇化金属氧化物叉指微型电极表面的金纳米材料混合用于诊断前列腺癌

前列腺癌是主要的健康负担，是男性第三大常见癌症。具有合适生物标志物的高精度检测和筛选方法可以显着降低死亡风险。前列腺特异性抗原 (PSA) 是有效且可接受的生物标志物，因为它在前列腺癌患者的生物体液中增加水平。本研究的重点是通过在叉指微型电极上使用金纳米颗粒 (GNP) 缀合的 PSA 特异性适体建立一种灵敏的 PSA 检测方法。GNP 允许在表面捕获更多数量的适体并增强 PSA 的相互作用。这种良好的检测方法可以在45[公式：见文]aM处测定PSA，灵敏度为30[公式：见文]aM。在 [公式：见文本] 处的回归曲线上，注意到从 60 到 2000 上午的线性范围；[公式：见正文]。此外，在人血清中添加 PSA 会随着 PSA 浓度的增加而增强当前的反应。这种测定方法有助于量化 PSA 水平并诊断不同阶段的前列腺癌。