Revisiting staining of biological samples for electron microscopy: perspectives for recent research,Materials Horizons

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Revisiting staining of biological samples for electron microscopy: perspectives for recent research
Materials Horizons ( IF 12.2 ) Pub Date : 2020-11-16 , DOI: 10.1039/d0mh01579b
Maren T Kuchenbrod ₁ , Ulrich S Schubert , Rainer Heintzmann , Stephanie Hoeppener

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This review revisits essential staining protocols for electron microscopy focussing on the visualization of active sites, i.e. enzymes, metabolites or proteins, in cells and tissues, which have been developed 50 to 60 years ago, however, never were established as standard protocols being used in electron microscopy in a routine fashion. These approaches offer numerous possibilities to expand the knowledge of cellular function and specifically address the localization of active compounds of these systems. It is our conviction, that many of these techniques are still useful, in particular when applied in conjunction with correlative light and electron microscopy. Revisiting specialized classical electron microscopy staining protocols for use in correlative microscopy is particularly promising, as some of these protocols were originally developed as staining methods for light microscopy. To account for this history, rather than summarizing the most recent achievements in literature, we instead first provide an overview of techniques that have been used in the past. While some of these techniques have been successfully implemented into modern microscopy techniques during recent years already, more possibilities are yet to be re-discovered and provide exciting new perspectives for their future use.

中文翻译：

重新审视电子显微镜生物样品的染色：近期研究的观点

这篇综述重新审视了电子显微镜的基本染色协议，重点是活性位点的可视化，即然而，在 50 至 60 年前开发的细胞和组织中的酶、代谢物或蛋白质从未被确立为常规方式用于电子显微镜的标准方案。这些方法提供了许多可能性来扩展细胞功能的知识，并专门解决这些系统的活性化合物的定位。我们坚信，其中许多技术仍然有用，特别是在与相关光学和电子显微镜结合使用时。重新审视用于相关显微镜的专业经典电子显微镜染色协议特别有希望，因为其中一些协议最初是作为光学显微镜的染色方法开发的。为了说明这段历史，我们不是总结最近的文学成就，而是首先概述过去使用的技术。尽管近年来其中一些技术已成功应用于现代显微镜技术，但仍有更多的可能性有待重新发现，并为它们的未来使用提供令人兴奋的新视角。

更新日期：2020-11-27

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