Rotator cuff tears (RCTs) often require reconstructive surgery. Tendon-bone healing is critical for the outcome of rotator cuff reconstruction, but the process of tendon-bone healing is complex and difficult. Mesenchymal stem cells (MSCs) are considered to be an effective method to promote tendon-bone healing. MSCs have strong paracrine, anti-inflammatory, immunoregulatory, and angiogenic potential. Recent studies have shown that MSCs achieve many regulatory functions through exosomes. The purpose of this study was to explore the role of bone marrow mesenchymal stem cell-derived exosomes (BMSC-Exos) in tendon-bone healing. Our study found that BMSC-Exos promote the proliferation, migration, and angiogenic tube formation of human umbilical vein endothelial cells (HUVECs). The mechanism by which BMSC-Exos achieve this may be through the regulation of the angiogenic signaling pathway. In addition, BMSC-Exos can inhibit the polarization of M1 macrophages and inhibit the secretion of proinflammatory factors by M1 macrophages. After rotator cuff reconstruction in rats, BMSC-Exos were injected into the tail vein to analyze their effect on the rotator cuff tendon-bone interface healing. It was confirmed that BMSC-Exos increased the breaking load and stiffness of the rotator cuff after reconstruction in rats, induced angiogenesis around the rotator cuff endpoint, and promoted growth of the tendon-bone interface. BMSC-Exos promote tendon-bone healing after rotator cuff reconstruction in rats by promoting angiogenesis and inhibiting inflammation.

中文翻译：

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骨髓间充质干细胞来源的外来体通过促进大鼠血管新生和调节M1巨噬细胞而促进肩袖腱-骨愈合

肩袖撕裂（RCT）通常需要重建手术。肌腱骨的愈合对于肩袖重建的结果至关重要，但肌腱骨的愈合过程既复杂又困难。间充质干细胞（MSCs）被认为是促进肌腱骨愈合的有效方法。MSC具有强大的旁分泌，抗炎，免疫调节和血管生成潜能。最近的研究表明，MSC通过外来体实现许多调节功能。这项研究的目的是探讨骨髓间充质干细胞衍生的外来体（BMSC-Exos）在肌腱-骨愈合中的作用。我们的研究发现BMSC-Exos促进人脐静脉内皮细胞（HUVEC）的增殖，迁移和血管生成管的形成。BMSC-Exos实现这一目标的机制可能是通过调节血管生成信号通路。此外，BMSC-Exos可以抑制M1巨噬细胞的极化，并抑制M1巨噬细胞分泌促炎因子。大鼠肩袖重建后，将BMSC-Exos注入尾静脉以分析其对肩袖腱-骨界面愈合的影响。已经证实，BMSC-Exos在大鼠重建后增加了肩袖的断裂负荷和刚度，诱导了肩袖端点周围的血管生成，并促进了肌腱-骨界面的生长。BMSC-Exos通过促进血管生成和抑制炎症反应，在大鼠肩袖重建后促进肌腱骨愈合。BMSC-Exos可以抑制M1巨噬细胞的极化，并抑制M1巨噬细胞分泌促炎因子。大鼠肩袖重建后，将BMSC-Exos注入尾静脉以分析其对肩袖腱-骨界面愈合的影响。已经证实，BMSC-Exos在大鼠重建后增加了肩袖的断裂负荷和刚度，诱导了肩袖端点周围的血管生成，并促进了肌腱-骨界面的生长。BMSC-Exos通过促进血管生成和抑制炎症反应，在大鼠肩袖重建后促进肌腱骨愈合。BMSC-Exos可以抑制M1巨噬细胞的极化，并抑制M1巨噬细胞分泌促炎因子。大鼠肩袖重建后，将BMSC-Exos注入尾静脉以分析其对肩袖腱-骨界面愈合的影响。已经证实，BMSC-Exos在大鼠重建后增加了肩袖的断裂负荷和刚度，诱导了肩袖端点周围的血管生成，并促进了肌腱-骨界面的生长。BMSC-Exos通过促进血管生成和抑制炎症反应，在大鼠肩袖重建后促进肌腱骨愈合。将BMSC-Exos注入尾静脉以分析其对肩袖腱-骨界面愈合的影响。已经证实，BMSC-Exos在大鼠重建后增加了肩袖的断裂负荷和刚度，诱导了肩袖端点周围的血管生成，并促进了肌腱-骨界面的生长。BMSC-Exos通过促进血管生成和抑制炎症反应，在大鼠肩袖重建后促进肌腱骨愈合。将BMSC-Exos注入尾静脉以分析其对肩袖腱-骨界面愈合的影响。已经证实，BMSC-Exos在大鼠重建后增加了肩袖的断裂负荷和刚度，诱导了肩袖端点周围的血管生成，并促进了肌腱-骨界面的生长。BMSC-Exos通过促进血管生成和抑制炎症反应，在大鼠肩袖重建后促进肌腱骨愈合。