Diabetic foot ulcer is the most costly and complex challenge for patients with diabetes. We hereby assessed the effectiveness of different preconditioned adipose-derived mesenchymal stem cells (AD-MSCs) and photobiomodulation protocols on treating an infected ischemic wound in type 1 diabetic rats. There were five groups of rats: (1) control, (2) control AD-MSCs [diabetic AD-MSCs were transplanted (grafted) into the wound bed], (3) AD-MSC + photobiomodulation in vivo (diabetic AD-MSCs were grafted into the wound, followed by in vivo PBM treatment), (4) AD-MSCs + photobiomodulation in vitro, and (5) AD-MSCs + photobiomodulation in vitro + in vivo. Diabetic AD-MSCs preconditioned with photobiomodulation had significantly risen cell function compared to diabetic AD-MSC. Groups 3 and 5 had significantly decreased microbial flora correlated to groups 1 and 2 (all, p = 0.000). Groups 2, 3, 4, and 5 had significantly improved wound closure rate (0.4, 0.4, 0.4, and 0.8, respectively) compared to group 1 (0.2). Groups 2–5 had significantly increased wound strength compared to group 1 (all p = 0.000). In most cases, group 5 had significantly better results than groups 2, 3, and 4. Preconditioning diabetic AD-MSCs with photobiomodulation in vitro plus photobiomodulation in vivo significantly hastened healing in the diabetic rat model of an ischemic infected delayed healing wound.

中文翻译：

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光生物调节预处理糖尿病干细胞移植加速糖尿病大鼠缺血性伤口愈合


糖尿病足溃疡是糖尿病患者面临的最昂贵、最复杂的挑战。我们在此评估了不同预处理的脂肪源性间充质干细胞 (AD-MSC) 和光生物调节方案在治疗 1 型糖尿病大鼠感染的缺血性伤口方面的有效性。大鼠分为五组：（1）对照组，（2）对照AD-MSCs[糖尿病AD-MSCs被移植（移植）到创面床]，（3）AD-MSC+体内光生物调节（糖尿病AD-MSCs）移植到伤口中，然后进行体内 PBM 处理），（4）AD-MSC + 体外光生物调节，以及（5）AD-MSC + 体外光生物调节 + 体内。与糖尿病 AD-MSC 相比，经过光生物调节预处理的糖尿病 AD-MSC 的细胞功能显着增强。与第 1 组和第 2 组相比，第 3 组和第 5 组的微生物菌群显着减少（全部，p = 0.000）。与第 1 组 (0.2) 相比，第 2、3、4 和 5 组的伤口闭合率显着提高 (分别为 0.4、0.4、0.4 和 0.8)。与第 1 组相比，第 2-5 组的伤口强度显着增加（所有 p = 0.000）。在大多数情况下，第 5 组的结果明显优于第 2、3 和 4 组。体外光生物调节加上体内光生物调节预处理糖尿病 AD-MSC 可显着加速糖尿病大鼠模型缺血性感染延迟愈合伤口的愈合。