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Polarization behavior of bone marrow-derived macrophages on charged P(VDF-TrFE) coatings
Biomaterials Science ( IF 5.8 ) Pub Date : 2020-11-14 , DOI: 10.1039/d0bm01604g Zhiying Wang 1, 2, 3, 4, 5 , Xuzhao He 1, 2, 3, 4, 5 , Bolin Tang 5, 6, 7, 8 , Xiaoyi Chen 3, 5, 9, 10, 11 , Lingqing Dong 3, 5, 9, 10, 11 , Kui Cheng 1, 2, 3, 4, 5 , Wenjian Weng 1, 2, 3, 4, 5
Biomaterials Science ( IF 5.8 ) Pub Date : 2020-11-14 , DOI: 10.1039/d0bm01604g Zhiying Wang 1, 2, 3, 4, 5 , Xuzhao He 1, 2, 3, 4, 5 , Bolin Tang 5, 6, 7, 8 , Xiaoyi Chen 3, 5, 9, 10, 11 , Lingqing Dong 3, 5, 9, 10, 11 , Kui Cheng 1, 2, 3, 4, 5 , Wenjian Weng 1, 2, 3, 4, 5
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The immune response of bone implants is closely related to the interaction between macrophages and biomaterial surfaces. In this work, the polarization behavior of mouse bone marrow-derived macrophages (BMDMs), including their morphology and expression of phenotypic markers, genes and cytokines, on charged surfaces with different potential intensities was systematically explored. We found that the charged surface could effectively promote BMDM polarization, and a higher potential intensity was conducive to the upregulation of the polarization of BMDMs into the M2 phenotype. Based on the analysis of the signaling pathways involved in integrins (αMβ2 and α5β1) and the potassium ion channel (Kv1.3), a possible underlying mechanism revealed that the integrin originated signaling pathways could more dominantly regulate macrophage polarization to the M2 phenotype. The present work therefore demonstrates that the surface charge, as a physicochemical property of material surfaces, could effectively regulate macrophage polarizations, which may provide an immunoregulation view for the surface design of biomaterials.
中文翻译：
骨髓衍生的巨噬细胞在带电P（VDF-TrFE）涂层上的极化行为
骨植入物的免疫反应与巨噬细胞和生物材料表面之间的相互作用密切相关。在这项工作中，系统地研究了小鼠骨髓衍生的巨噬细胞（BMDM）的极化行为，包括它们的形态和表型标记，基因和细胞因子在具有不同潜在强度的带电表面上的表达。我们发现带电的表面可以有效地促进BMDM极化，较高的电位强度有助于BMDMs的极化向上调节为M2表型。基于对整合素（αMβ2和α5β1）和钾离子通道（Kv1.3）涉及的信号通路的分析，可能的潜在机制揭示了整合素起源的信号传导途径可以更主要地将巨噬细胞极化调节为M2表型。因此，本工作证明表面电荷作为材料表面的物理化学性质，可以有效地调节巨噬细胞的极化，这可以为生物材料的表面设计提供免疫调节的观点。
更新日期：2020-12-16
中文翻译：
骨髓衍生的巨噬细胞在带电P（VDF-TrFE）涂层上的极化行为
骨植入物的免疫反应与巨噬细胞和生物材料表面之间的相互作用密切相关。在这项工作中，系统地研究了小鼠骨髓衍生的巨噬细胞（BMDM）的极化行为，包括它们的形态和表型标记，基因和细胞因子在具有不同潜在强度的带电表面上的表达。我们发现带电的表面可以有效地促进BMDM极化，较高的电位强度有助于BMDMs的极化向上调节为M2表型。基于对整合素（αMβ2和α5β1）和钾离子通道（Kv1.3）涉及的信号通路的分析，可能的潜在机制揭示了整合素起源的信号传导途径可以更主要地将巨噬细胞极化调节为M2表型。因此，本工作证明表面电荷作为材料表面的物理化学性质，可以有效地调节巨噬细胞的极化，这可以为生物材料的表面设计提供免疫调节的观点。
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