The kinetics of the formation of the light orange–coloured complex [Ru(CN)₅D-PA]³⁻ are studied spectrophotometrically through substitution of a coordinated H₂O molecule in aquapentacyanoruthenate(II) [Ru(CN)₅OH₂]³⁻ by interaction with D-penicillamine [D-PA] in aqueous medium at 490 nm (λ_max of [Ru(CN)₅D-PA]³⁻). The reaction is monitored under pseudo-first-order conditions, taking [D-PA] in excess over [Ru(CN)₅OH₂³⁻]. The effects of various reaction parameters on the rate of the reaction are investigated. Experimental observations reveal that the variation in [D-PA] obeyed the first-order rate law while it is found to be invariant with [Ru(CN)₅OH₂³⁻] in the whole experimental range. With ionic strength variation, as the reaction advances a decrease in the reaction rate is noticed. The product stoichiometry is assigned as 1:1. The ease of substitution at an H₂O molecule in [Ru(CN)₅OH₂³⁻] is considered on the basis of the electronic effect generated through interactions of the M–OH₂ bond. A provisional mechanism based on the calculated results is proposed based on the slowest step of the reaction.

通过取代水合戊二酸钌（II）[Ru（CN）₅ OH ₂ ]中配位的H ₂ O分子的分光光度法研究了浅橙色络合物[Ru（CN）₅ D-PA] ^3-的形成动力学。^3-通过用d青霉胺相互作用[d-PA]中在490nm（λ水性介质_最大的[Ru（CN）的₅ d-PA] ^3- ）。该反应伪一阶条件下监测，取[d-PA]在过量的[Ru（CN）₅ OH ₂ ^3-]。研究了各种反应参数对反应速率的影响。实验观察表明，在整个实验范围内，[D-PA]的变化服从一阶速率定律，而[Ru（CN）₅ OH ₂ ^3-则是不变的。随着离子强度的变化，随着反应的进行，注意到反应速率的降低。产物化学计量为1：1。根据通过M–OH ₂相互作用产生的电子效应，考虑[Ru（CN）₅ OH ₂ ^3- ]中H ₂ O分子的易取代性键。基于反应最慢的步骤，提出了一种基于计算结果的临时机制。