Recently, we published a manuscript in Langmuir analyzing the detachment of kinesin-propelled microtubules in gliding assays. We discovered a calibration error in the interference reflection microscopy (IRM) measurements. This calibration error has no effect on the conclusions in this article since it occurred only in the IRM measurements, which were performed to demonstrate that photobleaching is not the reason for microtubule detachment in the fluorescence microscopy experiments. The correct average distance traveled before detachment under IRM imaging (page 7904, first line under Figure 4) is 19 ± 9 mm. This is not significantly lower than the distance traveled before detachment under fluorescence imaging: 30 ± 10 mm. In the Supporting Information, the scale bar in Supporting Figures 6 and 7 should read 6.67 μm. Supporting Table 3 (labeled “Table 1” here) should be the following: The FOV for the fluorescence microscopy is 6708 μm², and that for the IRM is 7876 μm². To determine the number of observed microtubules in IRM microscopy, we counted one-third of an FOV. The calculation of the average distance traveled before detachment under IRM imaging should read This article has not yet been cited by other publications.

中文翻译：

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对“滑行运动分析中的微管分离限制了驱动蛋白驱动的分子梭的性能”的更正

最近，我们在Langmuir发表了手稿在滑动实验中分析驱动蛋白推动的微管的分离。我们在干涉反射显微镜（IRM）测量中发现了校准误差。该校准误差对本文的结论没有影响，因为它仅发生在IRM测量中，这表明荧光漂白不是荧光显微镜实验中微管脱离的原因。在IRM成像下进行分离之前，正确的平均行进距离（第7904页，图4中的第一行）为19±9 mm。这并不显着低于荧光成像分离前的移动距离：30±10 mm。在“支持信息”中，支持图6和7中的比例尺应为6.67μm。支持表3（此处标记为“表1”）应为以下内容：²，并且对于所述IRM是7876微米²。为了确定在IRM显微镜下观察到的微管的数量，我们计算了FOV的三分之一。在IRM成像下分离前平均行进距离的计算方法应为：本文尚未被其他出版物引用。