Natural elicitors derived from pathogenic microorganisms represent an ecologic strategy to achieve resistance in plants against diseases. Glucosylceramides (GlcCer) are classified as neutral glycosphingolipids. GlcCer were isolated and purified from Fusarium oxysporum mycelium. F. oxysporum is a plant pathogenic fungus, abundant in soil and causing severe losses in economically important crops such as corn, tobacco, banana, cotton and passion fruit. In this study we evaluate the capacity of GlcCer in inducing resistance in N. tabacum cv Xanthi plants against Tobacco mosaic virus (TMV). Spraying tobacco plants with GlcCer before virus infection reduced the incidence of necrotic lesions caused by TMV. In addition, plants already infected with the virus showed a reduction in hypersensitive response (HR) lesions after GlcCer treatment, suggesting an antiviral effect of GlcCer. Our investigations showed that GlcCer stimulates the early accumulation of H₂O₂ and superoxide radicals. In addition, the expression of PR-1 (pathogenesis-related 1, with suggested antifungal action), PR-2 (β-1,3-glucanase), PR-3 (Chitinase), PR-5 (Osmotin), PAL (Phenylalanine ammonia-lyase), LOX (Lipoxygenase) and POX (Peroxidase) genes was highly induced after treatment of tobacco plants with GlcCer and induction levels remained high throughout a period of 6 to 120 hours. Our experiments demonstrate that GlcCer induces resistance in tobacco plants against infection by TMV.

源自病原微生物的天然诱导子代表了一种实现植物抗病性的生态策略。葡萄糖神经酰胺 (GlcCer) 被归类为中性糖鞘脂。 GlcCer 从尖孢镰刀菌菌丝体中分离纯化。 F 。尖孢菌是一种植物病原真菌，在土壤中大量存在，对玉米、烟草、香蕉、棉花和百香果等重要经济作物造成严重损失。在本研究中，我们评估了 GlcCer 诱导N抗性的能力。 tabacum cv Xanthi 植物抗烟草花叶病毒(TMV)。在病毒感染前用 GlcCer 喷洒烟草植物可减少 TMV 引起的坏死病斑的发生率。此外，已经感染病毒的植物在 GlcCer 处理后表现出过敏反应 (HR) 病变的减少，表明 GlcCer 具有抗病毒作用。我们的研究表明，GlcCer 会刺激 H ₂ O ₂和超氧自由基的早期积累。此外，PR-1（发病机制相关 1，建议具有抗真菌作用）、PR-2（β-1,3-葡聚糖酶）、PR-3（几丁质酶）、PR-5（渗透压素）、PAL（用 GlcCer 处理烟草植物后，苯丙氨酸解氨酶、LOX（脂氧合酶）和 POX（过氧化物酶）基因被高度诱导，并且诱导水平在 6 至 120 小时内保持较高水平。我们的实验表明，GlcCer 诱导烟草植物抵抗 TMV 感染。