Coelenterazine Analogue with Human Serum Albumin-Specific Bioluminescence,Bioconjugate Chemistry

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Coelenterazine Analogue with Human Serum Albumin-Specific Bioluminescence
Bioconjugate Chemistry ( IF 4.0 ) Pub Date : 2020-11-25 , DOI: 10.1021/acs.bioconjchem.0c00536
Ryo Nishihara _{1,

2} , Kazuki Niwa ₁ , Tatsunosuke Tomita _{1,

2} , Ryoji Kurita _{1,

2,

3}

Affiliation

A synthetic luciferin comprising an imidazopyrazinone core, named HuLumino1, was designed to generate specific bioluminescence with human serum albumin (HSA) in real serum samples. HuLumino1 was developed by attaching a methoxy-terminated alkyl chain to C-6 of coelenterazine and by eliminating a benzyl group at C-8. HSA levels were quantified within 5% error margins of an enzyme-linked immunosorbent assay without the need for any sample pretreatments because of the high specificity of HuLumino1.

中文翻译：

腔肠素类似物与人血清白蛋白特异性生物发光

设计了包含咪唑并吡嗪酮核心的合成萤光素，名为HuLumino 1，可在真实血清样品中与人血清白蛋白（HSA）产生特定的生物发光。HuLumino 1是通过在腔肠素的C-6上连接一个甲氧基封端的烷基链并消除C-8上的一个苄基而开发的。由于HuLumino 1的高特异性，无需进行任何样品预处理，即可在酶联免疫吸附测定的5％误差范围内对HSA水平进行定量。

更新日期：2020-12-16

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