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Simultaneous Detection of Human Serum Albumin and Sulfur Dioxide in Living Cells Based on a Catalyzed Michael Addition Reaction
Analytical Chemistry ( IF 6.7 ) Pub Date : 2020-11-25 , DOI: 10.1021/acs.analchem.0c03806
Zengqiang Liang 1 , Yuanqiang Sun 1 , Huajin Zeng 2 , Kai Sun 1 , Ran Yang 1 , Zhaohui Li 1 , Ke Zhang 3 , Xiaolan Chen 1 , Lingbo Qu 1
Affiliation  

As vital important bioactive species, human serum albumin (HSA) and sulfur dioxide (SO2) are essential molecules in the organisms and act a pivotal part in many biological events. Although studies have shown that SO2-induced HSA radicals can cause oxidative damage, the underlying mechanism of the synergistic effect of HSA and SO2 in various diseases is obscure, mainly because of the lack of powerful tools that can simultaneously detect HSA and SO2 in living systems. In this work, we report a novel single-site, double-sensing fluorescent probe 1 for the simultaneous detection of HSA and SO2. The probe is based on our finding that HSA can catalyze a Michael addition reaction between the probe and SO2, which induces a change in fluorescence. Probe 1 can effectively entered the endoplasmic reticulum and can be used to image exogenously introduced and de novo synthesis of HSA in endoplasmic reticulum. Furthermore, the simultaneous detection of HSA and SO2 was realized for the first time with probe 1. More important, we observed that HSA still retains its activity to catalyze the Michael addition reaction of 1 and SO2 in living cells, which may provide a significant boost in the study of the role of HSA in medicine and pharmacy.

中文翻译:

催化迈克尔加成反应同时检测活细胞中人血清白蛋白和二氧化硫

作为至关重要的重要生物活性物种,人血清白蛋白(HSA)和二氧化硫(SO 2)是生物体中必不可少的分子,并且在许多生物事件中起着举足轻重的作用。尽管研究表明SO 2诱导的HSA自由基可引起氧化损伤,但在各种疾病中HSA和SO 2协同作用的潜在机制尚不清楚,这主要是由于缺乏可同时检测HSA和SO 2的强大工具在生命系统中。在这项工作中,我们报告了用于同时检测HSA和SO 2的新型单点,双感应荧光探针1。该探针基于我们的发现,即HSA可以催化探针与SO 2之间的迈克尔加成反应,从而引起荧光变化。探针1可以有效地进入内质网,并且可以用于对内质网中HSA的外源导入和从头合成成像。此外,首次使用探针1实现了HSA和SO 2的同时检测。更重要的是,我们观察到HSA仍然保留了其在活细胞中催化1和SO 2的迈克尔加成反应的活性,这可能在研究HSA在医学和药学中的作用方面提供了重要的推动力。
更新日期:2020-12-15
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