The transgenic Liberty Link® (LL) soybean is tolerant to glufosinate, conferred by the enzyme phosphinothricin acetyltransferase (PAT), which is encoded by the pat gene from Streptomyces viridochromogenes. Because symptoms of injury can be observed in soybean [Glycine max (L.) Merr.] plants in some situations, this study evaluated the effects of rates of glufosinate on agronomic performance; quality of LL soybean seeds; and the ammonia, glufosinate, and N-acetyl-l-glufosinate concentration (NAG) in soybeans with and without the pat gene after application of increasing glufosinate rates. Field and greenhouse experiments were conducted; the first evaluated the selectivity of glufosinate in LL soybeans, and the second evaluated the metabolic changes in soybeans with (LL) and without (RR2) the pat gene, after application of glufosinate. For fieldwork, application of glufosinate at rates up to four times the maximum recommended caused initial injury symptoms (up to 38.5%) in LL soybean plants. However, no negative effect was found on seed quality and agronomic performance of LL plants, including yield. This shows the selectivity of glufosinate promoted by pat gene insertion for application in POST (V4), in LL soybean. For the greenhouse experiment, it was concluded that the LL soybean plants presented high glufosinate metabolism, lower ammonia concentration, and no reduction in dry matter, in comparison with RR2 soybean, after application of high rates of glufosinate.

中文翻译：

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pat基因大豆在施用草铵膦后的代谢变化、农艺性能和种子品质

转基因自由链接®(LL) 大豆对草铵膦具有耐受性，这是由草铵膦乙酰转移酶 (PAT) 赋予的，该酶由拍基因来自绿色发色链霉菌. 因为在大豆中可以观察到受伤的症状 [最大甘氨酸(L.) Merr.] 植物在某些情况下，本研究评估了草铵膦用量对农艺性能的影响；LL大豆种子的质量；以及氨、草铵膦和ñ-乙酰基-l-大豆中的草铵膦浓度（NAG）拍应用增加草铵膦率后的基因。进行了田间和温室实验；第一个评估了 LL 大豆中草铵膦的选择性，第二个评估了有 (LL) 和没有 (RR2) 的大豆中的代谢变化拍基因，在应用草铵膦后。在田间工作中，草铵膦的施用率高达推荐最大值的四倍，导致 LL 大豆植物的初始损伤症状（高达 38.5%）。然而，没有发现对LL植物的种子质量和农艺性能（包括产量）产生负面影响。这表明草铵膦的选择性由拍用于 POST (V4)、LL 大豆的基因插入。对于温室试验，得出的结论是，与 RR2 大豆相比，在施用高草铵膦后，LL 大豆植物表现出较高的草铵膦代谢，较低的氨浓度，并且没有减少干物质。