Larix olgensis or larch is an economically important coniferous tree species with rapid growth in the early stages, strong adaptability, and a short time to harvest. The genetic improvement of larch has garnered considerable attention in recent years for reclaiming timber forests. However, traditional breeding methods are largely ineffective for achieving rapid genetic improvement of L. olgensis. Studies show that the efficiency of plant regeneration can be improved by optimizing somatic embryogenesis. On this basis, we devised a stable, fast and efficient Agrobacterium-mediated genetic transformation method using suspended embryogenic calluses as explants and β-glucuronidase as the reporter. We evaluated the effects of the Agrobacterium load, co-culture period, and addition of acetosyringone and transformant screening antibiotic on the transformation efficiency. In addition, we tested the pCAMBIA 1300-PtHCA 2-1 promoter-GUS binary expression vector, which contains the GUS gene ORF under the control of Populus trichocarpa high cambial activity PtHCA 2-1 promoter, and observed the tissue-specific expression of the GUS gene in the somatic embryos of transgenic larch. This novel technique can not only accelerate the generation of superior transgenic strains of L. olgensis but also aid in future gene functional studies.

落叶松落叶松是具有重要经济意义的针叶树种，早期生长迅速，适应性强，收获时间短。近年来，落叶松的遗传改良在开垦木材森林方面引起了相当大的关注。但是，传统的育种方法在很大程度上无法实现遗传改良。香菇。研究表明，通过优化体细胞胚发生可以提高植物再生的效率。在此基础上，我们设计了稳定，快速，高效的农杆菌悬浮的胚性愈伤组织为外植体，以β-葡萄糖醛酸苷酶为报告基因的介导的遗传转化方法。我们评估了农杆菌负载量，共培养时间，以及加入乙酰丁香酮和转化体筛选抗生素对转化效率的影响。此外，我们测试了pCAMBIA 1300-铂HCA 2-1启动子-GUS二元表达载体，其中包含在控制下的GUS基因ORF 毛果杨 冈比亚活动活跃 铂HCA 2-1启动子，并观察到GUS基因在转基因落叶松体细胞胚中的组织特异性表达。这项新技术不仅可以加速抗性的优良转基因菌株的产生香菇 而且还有助于将来的基因功能研究。