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Simultaneously Detecting Monoamine Oxidase A and B in Disease Cell/Tissue Samples Using Paper-Based Devices
ACS Applied Bio Materials ( IF 4.6 ) Pub Date : 2020-11-25 , DOI: 10.1021/acsabm.0c01288 Haixiao Fang 1 , Meirong Wu 1 , Wenhui Ji 1 , Limin Wang 2 , Yipei Chen 2 , Ding Chen 1 , Naidi Yang 1 , Qiong Wu 1 , Changmin Yu 1 , Jie Liu 1 , Jinhua Liu 1 , Hua Bai 2 , Bo Peng 2 , Xiao Huang 1 , Hai-Dong Yu 1, 2 , Lin Li 1
ACS Applied Bio Materials ( IF 4.6 ) Pub Date : 2020-11-25 , DOI: 10.1021/acsabm.0c01288 Haixiao Fang 1 , Meirong Wu 1 , Wenhui Ji 1 , Limin Wang 2 , Yipei Chen 2 , Ding Chen 1 , Naidi Yang 1 , Qiong Wu 1 , Changmin Yu 1 , Jie Liu 1 , Jinhua Liu 1 , Hua Bai 2 , Bo Peng 2 , Xiao Huang 1 , Hai-Dong Yu 1, 2 , Lin Li 1
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As enzymes in the outer membrane of the mitochondrion, monoamine oxidases (MAOs) can catalyze the oxidative deamination of monoamines in the human body. According to different substrates, MAOs can be divided into MAO-A and MAO-B. The imbalance of the MAO-A is associated with neurological degeneration, while excess MAO-B activity is closely connected with Parkinson’s disease (PD) and Alzheimer’s disease (AD); therefore, detection of MAOs is of great significance for the diagnosis and treatment of these diseases. This work reports the multiplexed detection of MAO-A and MAO-B using paper-based devices based on chemiluminescence (CL). The detection limits were 5.01 pg/mL for MAO-A and 8.50 pg/mL for MAO-B in human serum. In addition, we used paper-based devices to detect MAOs in human cells and tissue samples and found that the results of paper-based detection and Western blotting (WB) showed the same trend. While only one antibody can be incubated on the same membrane by WB, multiple antibodies incubated on the same paper enabled simultaneous detection of MAO-A and MAO-B by paper-based devices. The paper-based assay could be used for preliminary early screening of clinical samples for MAOs and can be extended as an alternative to WB for multiplexed detection of various proteins in disease cell or tissue samples.
中文翻译：
使用纸基设备同时检测疾病细胞/组织样本中的单胺氧化酶 A 和 B
作为线粒体外膜的酶，单胺氧化酶（MAOs）可以催化人体内单胺的氧化脱氨作用。根据不同的底物，MAOs可分为MAO-A和MAO-B。MAO-A的失衡与神经退行性变有关，而MAO-B活性过度则与帕金森病（PD）和阿尔茨海默病（AD）密切相关；因此，MAOs的检测对于这些疾病的诊断和治疗具有重要意义。这项工作报告了使用基于化学发光 (CL) 的纸基设备对 MAO-A 和 MAO-B 进行多重检测。人血清中 MAO-A 的检测限为 5.01 pg/mL，MAO-B 的检测限为 8.50 pg/mL。此外，我们使用纸基设备检测人体细胞和组织样本中的 MAO，发现纸基检测和蛋白质印迹 (WB) 的结果显示出相同的趋势。虽然 WB 只能在同一膜上孵育一种抗体，但在同一张纸上孵育的多种抗体可以通过纸基设备同时检测 MAO-A 和 MAO-B。基于纸张的测定可用于临床样品的 MAO 初步早期筛查，并可扩展为替代 WB 的方法，用于对疾病细胞或组织样品中的各种蛋白质进行多重检测。
更新日期：2020-11-25
中文翻译：
使用纸基设备同时检测疾病细胞/组织样本中的单胺氧化酶 A 和 B
作为线粒体外膜的酶，单胺氧化酶（MAOs）可以催化人体内单胺的氧化脱氨作用。根据不同的底物，MAOs可分为MAO-A和MAO-B。MAO-A的失衡与神经退行性变有关，而MAO-B活性过度则与帕金森病（PD）和阿尔茨海默病（AD）密切相关；因此，MAOs的检测对于这些疾病的诊断和治疗具有重要意义。这项工作报告了使用基于化学发光 (CL) 的纸基设备对 MAO-A 和 MAO-B 进行多重检测。人血清中 MAO-A 的检测限为 5.01 pg/mL，MAO-B 的检测限为 8.50 pg/mL。此外，我们使用纸基设备检测人体细胞和组织样本中的 MAO，发现纸基检测和蛋白质印迹 (WB) 的结果显示出相同的趋势。虽然 WB 只能在同一膜上孵育一种抗体，但在同一张纸上孵育的多种抗体可以通过纸基设备同时检测 MAO-A 和 MAO-B。基于纸张的测定可用于临床样品的 MAO 初步早期筛查，并可扩展为替代 WB 的方法，用于对疾病细胞或组织样品中的各种蛋白质进行多重检测。
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