Current commercially available methods for reliably detecting antibodies against severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) remain expensive and inaccessible due to the need for whole-blood collection by highly trained phlebotomists using personal protective equipment (PPE). We have evaluated an antibody detection approach using the OraSure Technologies oral antibody collection device (OACD) and their proprietary SARS-CoV-2 total antibody detection enzyme-linked immunosorbent assay (ELISA). We found that the OraSure test for total antibody detection in oral fluid had comparable sensitivity and specificity to commercially available serum-based ELISAs for SARS-CoV-2 antibody detection while allowing for a more accessible form of specimen collection with the potential for self-collection.

中文翻译：

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快速收集装置在口腔液中检测SARS-CoV-2抗体

由于需要由训练有素的抽血者使用个人防护设备（PPE）进行全血收集，因此目前用于可靠检测严重急性呼吸综合征冠状病毒2（SARS-CoV-2）抗体的可商购方法仍然昂贵且难以获得。我们评估了使用OraSure Technologies口服抗体收集装置（OACD）及其专有的SARS-CoV-2总抗体检测酶联免疫吸附测定（ELISA）的抗体检测方法。我们发现，用于口服液中总抗体检测的OraSure测试与用于SARS-CoV-2抗体检测的市售基于血清的ELISA具有相当的敏感性和特异性，同时允许更简便的样本收集形式，具有自我收集的潜力。