The high demand of sea cucumbers in international markets, mostly based in Asia, has left a deep impact on the natural stock of many species, leading to an investment in aquaculture techniques in order to mitigate these impacts. This study aimed to determine the effects of stock density in rearing broodstock of Holothuria (Panningoturia) forskali Delle Chiaje, 1823, a common species in the Mediterranean Sea and eastern Atlantic with commercial value. To do so, two different density treatments were selected: 1 kg/m² and 2 kg/m². The trial took place over five months and individuals were fed a mixture of frozen microalgae (Tetraselmis sp. and Phaeodactylum tricornutum). At the end of the trial, the effects of stock density on the individuals’ condition were assessed by analysing different variables, namely their fresh and gutted weight, evisceration and mortality rates, gonadosomatic index and oocyte size and even biochemical content, such as protein and lipid content in muscle bands. No significant differences were found between density treatments regarding any of the parameters assessed, aside from mean oocyte diameter. Individuals from the lower‐density treatment presented a smaller mean oocyte diameter, supplied with fewer oocytes, than individuals in both the higher‐density treatment and the baseline group. Regardless of this one difference, all trial individuals were able to maintain their elevated gonadal developmental stage for a full period of five months past their reproductive peak, with female holothurians subjected to the higher‐density treatment showing signs of increasing vitellogenic reserves, a considerable advantage in aquaculture broodstock rearing.

中文翻译：

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放养密度对福寿螺亲鱼生理性状的影响

国际市场对海参的需求旺盛，主要是亚洲市场，对许多物种的自然资源产生了深远的影响，因此需要对水产养殖技术进行投资，以减轻这些影响。这项研究的目的是确定在饲养的亲鱼股票密度的影响玉足（Panningoturia）forskali阿尔Chiaje，1823年，在地中海和大西洋东部具有商业价值的一种常见的物种。为此，选择了两种不同的密度处理：1 kg / m ²和2 kg / m ²。该试验历时五个月，分别向个体饲喂冷冻微藻（Tetraselmis sp。和Phaeodyylum tricornutum）的混合物。）。在试验结束时，通过分析不同的变量来评估种群密度对个体状况的影响，这些变量包括其新鲜和去内脏的体重，内脏和死亡率，性腺体指数和卵母细胞大小，甚至是生化成分，例如蛋白质和蛋白质。肌肉带中的脂质含量。除平均卵母细胞直径外，在密度治疗之间，在评估的任何参数上均未发现显着差异。与高密度治疗组和基线组相比，低密度治疗组的个体平均卵母细胞直径较小，卵母细胞供应较少。不管这一差异如何，所有受试个体都能够在其生殖高峰之后的五个月内维持较高的性腺发育阶段，