Molecular investigation of adequate sources of mesenchymal stem cells for cell therapy of COVID‐19‐associated organ failure,STEM CELLS Translational Medicine

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Molecular investigation of adequate sources of mesenchymal stem cells for cell therapy of COVID‐19‐associated organ failure
STEM CELLS Translational Medicine ( IF 5.4 ) Pub Date : 2020-11-25 , DOI: 10.1002/sctm.20-0189
Christophe Desterke _{1,

2} , Frank Griscelli _{1,

2,

3} , Jusuf Imeri ₁ , Paul Marcoux ₁ , Thomas Lemonnier ₁ , Theodoros Latsis _{1,

2} , Ali G Turhan _{1,

2,

4} , Annelise Bennaceur-Griscelli _{1,

2,

4}

Affiliation

The use of mesenchymal stem cells (MSC) derived from several sources has been suggested as a major anti‐inflammation strategy during the recent outbreak of coronavirus‐19 (COVID‐19). As the virus enters the target cells through the receptor ACE2, it is important to determine if the MSC population transfused to patients could also be a target for the virus entry. We report here that ACE2 is highly expressed in adult bone marrow, adipose tissue, or umbilical cord‐derived MSC. On the other hand, placenta‐derived MSC express low levels of ACE2 but only in early passages of cultures. MSC derived from human embryonic stem cell or human induced pluripotent stem cells express also very low levels of ACE2. The transcriptome analysis of the MSCs with lowest expression of ACE2 in fetal‐like MSCs is found to be associated in particularly with an anti‐inflammatory signature. These results are of major interest for designing future clinical MSC‐based stem cell therapies for severe COVID‐19 infections.

中文翻译：

用于 COVID-19 相关器官衰竭细胞治疗的充足间充质干细胞来源的分子研究

在最近爆发的冠状病毒-19 (COVID-19) 期间，已建议使用来自多种来源的间充质干细胞 (MSC) 作为主要的抗炎策略。当病毒通过受体 ACE2 进入靶细胞时，重要的是确定输血给患者的 MSC 群是否也可能成为病毒进入的目标。我们在此报告 ACE2 在成人骨髓、脂肪组织或脐带衍生的 MSC 中高度表达。另一方面，胎盘来源的 MSC 表达低水平的 ACE2，但只在培养的早期传代中表达。源自人类胚胎干细胞或人类诱导多能干细胞的 MSC 也表达非常低水平的 ACE2。发现胎儿样 MSC 中 ACE2 表达最低的 MSC 的转录组分析尤其与抗炎特征相关。这些结果对于设计未来针对严重 COVID-19 感染的基于 MSC 的临床干细胞疗法具有重要意义。

更新日期：2020-11-25

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