Biofouling represents an important limitation in photobioreactor cultures. The biofouling propensity of different materials (polystyrene, borosilicate glass, polymethyl methacrylate, and polyethylene terephthalate glycol‐modified) and coatings (two spray‐applied and nanoparticle‐based superhydrophobic coatings and a hydrogel‐based fouling release coating) was evaluated by means of a short‐term protein test, using bovine serum albumin (BSA) as a model protein, and by the long‐term culture of the marine microalga Nannochloropsis gaditana under practical conditions. The results from both methods were similar, confirming that the BSA test predicts microalgal biofouling on surfaces exposed to microalgae cultures whose cells secrete macromolecules, such as proteins, with a high capacity for forming a conditioning film before cell adhesion. The hydrogel‐based coating showed significantly reduced BSA and N. gaditana adhesion, whereas the other surfaces failed to control biofouling. Microalgal biofouling was associated with an increased concentration of sticky extracellular proteins at low N/P ratios (below 15).

中文翻译：

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不同培养基 N/P 比下，微拟球藻微藻培养物中细胞外蛋白介导的长期生物污染形成

生物污染是光生物反应器培养的一个重要限制。不同材料（聚苯乙烯、硼硅酸盐玻璃、聚甲基丙烯酸甲酯和聚对苯二甲酸乙二醇酯乙二醇改性）和涂层（两种喷涂和纳米颗粒基超疏水涂层和水凝胶基污垢释放涂层）的生物污染倾向通过一种短期蛋白试验，以牛血清白蛋白（BSA）为模型蛋白，通过海洋微藻Nannochloropsis gaditana的长期培养在实际条件下。两种方法的结果相似，证实 BSA 测试可预测暴露于微藻培养物的表面上的微藻生物污垢，这些培养物的细胞分泌大分子（如蛋白质），具有在细胞粘附前形成调理膜的高能力。基于水凝胶的涂层显着降低了 BSA 和N. gaditana的粘附性，而其他表面未能控制生物污染。在低 N/P 比（低于 15）下，微藻生物污染与粘性细胞外蛋白浓度增加有关。