Glycated albumin (GA) is rapidly emerging as a robust biomarker for screening and monitoring of diabetes. To facilitate its rapid, point‐of‐care measurements, a label‐free surface‐enhanced Raman spectroscopy (SERS) sensing platform is reported that leverages the specificity of molecular vibrations and signal amplification on silver‐coated silicon nanowires (Ag/SiNWs) for highly sensitive and reproducible quantification of GA. The simulations and experimental measurements demonstrate that the disordered orientation of the nanowires coupled with the wicking of the analyte molecules during the process of solvent evaporation facilitates molecular trapping at the generated plasmonic hotspots. Highly sensitive detection of glycated albumin is shown with the ability to visually detect spectral features at as low as 500 × 10⁻⁹ m, significantly below the physiological range of GA in body fluids. Combined with chemometric regression models, the spectral data recorded on the Ag/SiNWs also allow accurate prediction of glycated concentration in mixtures of glycated and non‐glycated albumin in proportions that reflect those in the bloodstream.

中文翻译：

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镀银无序硅纳米线通过分子捕获和等离子体热点形成提供高灵敏度的无标记糖化白蛋白检测

糖化白蛋白 (GA) 正在迅速成为筛查和监测糖尿病的强大生物标志物。为了促进其快速的即时测量，据报道，一种无标记表面增强拉曼光谱 (SERS) 传感平台利用了银涂层硅纳米线 (Ag/SiNW) 上分子振动和信号放大的特异性，用于高度灵敏和可重复的 GA 定量。模拟和实验测量表明，纳米线的无序取向与溶剂蒸发过程中分析物分子的芯吸相结合，有助于在产生的等离子体热点处进行分子捕获。糖化白蛋白的高灵敏度检测显示出能够在低至 500 × 10 ^{-9的条件下目视检测光谱特征} m，明显低于体液中 GA 的生理范围。结合化学计量回归模型，记录在 Ag/SiNW 上的光谱数据还可以准确预测糖化和非糖化白蛋白混合物中的糖化浓度，其比例可反映血流中的糖化白蛋白。