Flexible Adipose‐Vascular Tissue Assembly Using Combinational 3D Printing for Volume‐Stable Soft Tissue Reconstruction,Advanced Healthcare Materials

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Flexible Adipose‐Vascular Tissue Assembly Using Combinational 3D Printing for Volume‐Stable Soft Tissue Reconstruction
Advanced Healthcare Materials ( IF 10.0 ) Pub Date : 2020-11-25 , DOI: 10.1002/adhm.202001693
Won‐Woo Cho _{1,

2} , Byoung Soo Kim _{2,

3} , Minjun Ahn _{1,

2} , Yeon Hee Ryu ₄ , Dong‐Heon Ha _{1,

5} , Jeong Sik Kong _{2,

6} , Jong‐Won Rhie ₇ , Dong‐Woo Cho _{1,

2}

Affiliation

A new concept, assembling cell‐laden tissue modules, is for the first time proposed for soft tissue engineering. Adipose‐vascular tissue modules composed of a synthetic polymer‐based substructure and customized bioinks using planar 3D cell printing are engineered. Such tissue modules are systematically assembled into a synthetic polymer‐based module holder fabricated with rotational 3D printing, resulting in the development of a flexible and volumetric tissue assembly. Whereas most of the previous studies about the construction of adipose tissue are limited to hypoxia, poor vascularization, rapid resorption, and mismatch in mechanical properties, it is aimed to realize the construction of nonhypoxic, flexible, and volume‐stable tissue assembly in this study. The significance of engineered tissue assembly is proven through various in vitro and in vivo evaluations. In particular, stable volume and remarkable neovascularization/adipogenesis are observed in the implanted assembly over four weeks. Interestingly, the size of newly formed lipid droplets and the remodeled morphology in the assembly are comparable to those in native adipose tissue. As far as it is known, this work is a first report suggesting a cell printing‐based tissue assembly for functional reconstruction of soft tissue.

中文翻译：

使用组合3D打印进行灵活的脂肪-血管组织组装，以稳定体积稳定的软组织重建

首次提出了一种新的概念，即组装装有细胞的组织模块，用于软组织工程。设计了由合成的基于聚合物的子结构和使用平面3D细胞打印定制的生物墨水组成的脂肪血管组织模块。此类组织模块被系统地组装到通过旋转3D打印制成的基于聚合物的合成模块支架中，从而开发出了灵活而体积庞大的组织组件。尽管先前有关脂肪组织构造的大多数研究仅限于缺氧，血管形成不良，快速吸收和机械特性不匹配，但本研究旨在实现无氧，柔性和体积稳定的组织组装的构造。。通过各种体外和体内评估证明了工程组织组装的重要性。特别是，在植入的组件中，在四个星期内观察到稳定的体积和显着的新血管形成/脂肪形成。有趣的是，组装中新形成的脂质滴的大小和重构的形态与天然脂肪组织中的脂质相当。据了解，这项工作是第一个报告，提出了基于细胞印刷的组织组装技术，用于软组织的功能重建。

更新日期：2020-11-25

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