Abstract Enzyme production as well as rice straw saccharification and fermentation were integrated to produce bioethanol in this study. Submerged fermentation of Na2CO3 pretreated rice straw to produce a complex enzyme for saccharification by Aspergillus fumigatus was performed. The major component of rice straw, that is cellulose, hemicellulose and lignin was almost completely degraded in 24 h. Using whole pretreated rice straw slurry as the substrate, the maximum concentrations of reducing sugar and ethanol were obtained with values of 63.6 g/L and 30.9 g/L, respectively, in a total hydrolysis and fermentation time at a substrate concentration of 8% (based on the original amount of rice straw). Fed-batch fermentation was employed for enzymolysis and fermentation of rice straw residue. Under a 32% total substrate concentration, 108.6 g/L ethanol was obtained in a total enzymolysis and fermentation time of 40 h. The results from different fermentation methods showed that the laccase produced by A. fumigatus in situ could effectively promote the enzymatic hydrolysis and fermentation through detoxifying the phenols produced during pretreatment and by the enzymatic hydrolysis processes.

中文翻译：

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用烟曲霉分泌的现场制造的酶对 Na2CO3 预处理的稻草进行糖化和解毒以提高生物乙醇产量

摘要 本研究将酶生产与稻草糖化发酵相结合生产生物乙醇。对 Na2CO3 预处理过的稻草进行深层发酵以生产烟曲霉糖化复合酶。稻草的主要成分，即纤维素、半纤维素和木质素在 24 小时内几乎完全降解。以整个预处理稻草浆为底物，在8%的底物浓度下，在总水解和发酵时间内，还原糖和乙醇的最大浓度分别为63.6 g/L和30.9 g/L（基于稻草的原始数量）。采用补料分批发酵法对稻草渣进行酶解发酵。在 32% 的总底物浓度下，108。在40小时的总酶解和发酵时间中获得6g/L乙醇。不同发酵方法的结果表明，烟曲霉原位产生的漆酶可以通过对预处理和酶解过程中产生的酚类物质进行解毒，从而有效地促进酶解和发酵。