当前位置: X-MOL 学术J. Plant Physiol. › 论文详情
Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)
MtFULc Controls Inflorescence Development by Directly Repressing MtTFL1 in Medicago truncatula
Journal of Plant Physiology ( IF 4.0 ) Pub Date : 2021-01-01 , DOI: 10.1016/j.jplph.2020.153329
Pengcheng Zhang , Ruiliang Wang , Xingchun Wang , Kirankumar S. Mysore , Jiangqi Wen , Yingying Meng , Xiaofeng Gu , Lifang Niu , Hao Lin

Flowering plants display a vast diversity of inflorescence architecture, which plays an important role in determining seed yield and fruit production. Unlike the model eudicot Arabidopsis thaliana that has simple inflorescences, most legume plants have compound types of inflorescences. Recent studies in the model legume species Pisum sativum and Medicago truncatula showed that the MADS-box transcription factors VEGETATIVE1/PsFRUITFULc/MtFRUITFULc (VEG1/PsFULc and MtFULc) are essential for the development of compound inflorescences by specifying the secondary inflorescence meristem identity. In this study, we report the isolation and characterization of two new mtfulc alleles by screening the M. truncatula Tnt1 insertion mutant collection. We found that MtFULc specifies M. truncatula secondary inflorescence meristem identity in a dose-dependent manner. Biochemical analysis and chromatin immunoprecipitation (ChIP) assays revealed that MtFULc acts as a transcriptional repressor to directly repress the expression of MtTFL1 through its promoter and 3' intergenic region. Comprehensive genetic analysis suggest MtFULc coordinates with the primary inflorescence meristem maintainer MtTFL1 and floral meristem regulator MtPIM to control M. truncatula inflorescence development. Our findings help to elucidate the mechanism of MtFULc-mediated regulation of secondary inflorescence meristem identity and provide insights into understanding the genetic regulatory network underlying compound inflorescence development in legumes.

中文翻译:

MtFULc 通过直接抑制苜蓿中的 MtTFL1 来控制花序发育

开花植物表现出多种多样的花序结构,这在决定种子产量和果实产量方面起着重要作用。与具有简单花序的模式双子叶植物拟南芥不同,大多数豆科植物具有复合类型的花序。最近对模式豆科植物豌豆和蒺藜苜蓿的研究表明,MADS-box 转录因子 VEGETATIVE1/PsFRUITFULc/MtFRUITFULc(VEG1/PsFULc 和 MtFULc)通过指定次生花序分生组织特征对复合花序的发育至关重要。在这项研究中,我们通过筛选 M. truncatula Tnt1 插入突变体集合来报告两个新的 mtfulc 等位基因的分离和表征。我们发现 MtFULc 指定了 M。truncatula 次生花序分生组织以剂量依赖性方式识别。生化分析和染色质免疫沉淀 (ChIP) 分析表明,MtFULc 作为转录抑制因子,通过其启动子和 3' 基因间区域直接抑制 MtTFL1 的表达。综合遗传分析表明,MtFULc 与初级花序分生组织维持者 MtTFL1 和花分生组织调节剂 MtPIM 协调控制 M. truncatula 花序发育。我们的研究结果有助于阐明 MtFULc 介导的次级花序分生组织特性调控机制,并为理解豆科植物复合花序发育的遗传调控网络提供见解。生化分析和染色质免疫沉淀 (ChIP) 分析表明,MtFULc 作为转录抑制因子,通过其启动子和 3' 基因间区域直接抑制 MtTFL1 的表达。综合遗传分析表明,MtFULc 与初级花序分生组织维持者 MtTFL1 和花分生组织调节剂 MtPIM 协调控制 M. truncatula 花序发育。我们的研究结果有助于阐明 MtFULc 介导的次级花序分生组织特性调控机制,并为理解豆科植物复合花序发育的遗传调控网络提供见解。生化分析和染色质免疫沉淀 (ChIP) 分析表明,MtFULc 作为转录抑制因子,通过其启动子和 3' 基因间区域直接抑制 MtTFL1 的表达。综合遗传分析表明,MtFULc 与初级花序分生组织维持者 MtTFL1 和花分生组织调节剂 MtPIM 协调控制 M. truncatula 花序发育。我们的研究结果有助于阐明 MtFULc 介导的次级花序分生组织特性调控机制,并为理解豆科植物复合花序发育的遗传调控网络提供见解。综合遗传分析表明,MtFULc 与初级花序分生组织维持者 MtTFL1 和花分生组织调节剂 MtPIM 协调控制 M. truncatula 花序发育。我们的研究结果有助于阐明 MtFULc 介导的次级花序分生组织特性调控机制,并为理解豆科植物复合花序发育的遗传调控网络提供见解。综合遗传分析表明,MtFULc 与初级花序分生组织维持者 MtTFL1 和花分生组织调节剂 MtPIM 协调控制 M. truncatula 花序发育。我们的研究结果有助于阐明 MtFULc 介导的次级花序分生组织特性调控机制,并为理解豆科植物复合花序发育的遗传调控网络提供见解。
更新日期:2021-01-01
down
wechat
bug