Aquaculture is a fast growing industry with its development hampered by bacterial diseases. Vibriosis caused by Harveyi clade strains is known for causing heavy loss especially in shrimp aquaculture farms. For farm treatment and pathogen spread management, veterinarians and researchers need reliable bacterial identification tools. A range of identification methods have been presented for Vibrio spp. in recent literature but little feedback on their performance have been made available to this day.

This study aims at comparing Vibrio spp. identification methods and providing guidance on their use.

Fifty farms were sampled and bacterial colonies were isolated using specific culture media before microscopic analysis and genomic profiling using ERIC-PCR. A preliminary identification step was carried out using MALDI-ToF mass spectrometry. Four methods were compared for strain identification on 14 newly isolated Harveyi clade Vibrio spp. strains: whole genome sequencing (digital DNA DNA Hybridization (dDDH)), 5 MLSA schemes, ferric uptake regulation (fur) and lecithin-dependent haemolysin (ldh) single gene based identification methods.

Apart from dDDH which is a reference method, no technique could identify all the isolates to the species level. The other tested techniques allowed a faster, cheaper but sub genus clade identification which can be interesting when absolute precision is not required. In this regard, MALDI-ToF and fur based identification seemed especially promising.

水产养殖是一个快速发展的行业，其发展受到细菌疾病的阻碍。众所周知，由Harveyi进化枝菌株引起的弧菌病会造成大量损失，尤其是在对虾养殖场中。对于农场处理和病原体传播管理，兽医和研究人员需要可靠的细菌识别工具。已经提出了一系列用于弧菌属的鉴定方法。在最近的文献中，但直到今天几乎没有关于他们表现的反馈。

本研究旨在比较弧菌属。识别方法并提供使用指南。

在使用 ERIC-PCR 进行显微分析和基因组分析之前，对 50 个农场进行了采样并使用特定的培养基分离了细菌菌落。使用 MALDI-ToF 质谱法进行初步鉴定步骤。对 14 个新分离的 Harveyi 进化枝弧菌的菌株鉴定进行了四种方法的比较。菌株：全基因组测序（数字 DNA DNA 杂交 (dDDH)）、5 种 MLSA 方案、铁摄取调节 ( fur ) 和卵磷脂依赖性溶血素 ( ldh ) 基于单基因的鉴定方法。

除了作为参考方法的 dDDH 外，没有任何技术可以在物种水平上识别所有分离株。其他测试技术允许更快、更便宜但亚属进化枝识别，这在不需要绝对精度时可能会很有趣。在这方面，MALDI-ToF 和基于毛皮的识别似乎特别有前途。