The oxidation-reduction potential (ORP) is an important factor in H₂ production via dark fermentation however its effect over microbial diversity in an acidogenic sludge has not, been well studied. This work studies the effect of ORP controlled by hydrogen peroxide and potassium ferricyanide on continuous hydrogen production and microbial diversity in an acidogenic sludge fed (HRT 12 h and pH 5.5) with glycerol. Results show that the more oxidizing ORP control environment (−540 mV) improves H₂ yield by 50–70% (0.31–0.51 molH₂/mol glycerol) over non-ORP control conditions. Oxidizing ORP values were shown to enrich microorganisms of the genus Clostridium, which have been linked to high H₂ yields. Therefore, controlling ORP in an acidogenic sludge was shown to directly modify microbial diversity at the genus level, and could likely to indirectly regulate metabolic function. Additionally, metabolic pathways were regulated by the kind of agent used.

氧化还原电位（ORP）是通过暗发酵生产H ₂的重要因素，但是，其对产酸污泥中微生物多样性的影响尚未得到充分研究。这项工作研究了过氧化氢和铁氰化钾控制的ORP对以甘油喂养的产酸污泥（HRT 12 h和pH 5.5）中连续产氢和微生物多样性的影响。结果表明，在非ORP控制条件下，氧化性更强的ORP控制环境（-540 mV）可将H ₂产量提高50–70％（0.31–0.51 molH ₂ / mol甘油）。显示氧化性ORP值可富集梭状芽胞杆菌属的微生物，这些微生物已与高H _2相关产量。因此，控制产酸污泥中的ORP可直接在属水平上改变微生物多样性，并可能间接调节代谢功能。另外，代谢途径受所用药剂种类的调节。