Four compounds, hericerin (1), isohericerinol A (2), N-de-phenylethyl isohericerin (3) and corallocin A (4) were isolated from the fruiting bodies of Hericium erinaceus, a lion’s mane mushroom (Hericiaceae). Among them, isohericerinol A (2) was newly reported in nature. Further investigation of the neurotrophic effect of isolated compounds demonstrated that isohericerinol A (2) strongly increased the nerve growth factor (NGF) production in C6 glioma cells followed by corallocin A (4) and hericerin (1). Increased NGF production by these compounds promoted the neurite outgrowth in N2a neuronal cells. Western blot analysis also showed the increased protein expression of NGF, brain-derived neurotrophic factor (BDNF) and synaptophysin (SYP) in C6-N2a cells. Taken together, our present study characterized the neurotrophic constituents of H. erinaceus, which may support the potential use of memory improvement.

四种化合物，hericerin（1），isohericerinol A（2），Ñ -de苯乙基isohericerin（3）和corallocin A（4）从子实体中分离猴头，狮子的鬃毛蘑菇（Hericiaceae）。其中，自然界中新报道了异黑菊醇A（2）。对分离出的化合物的神经营养作用的进一步研究表明，异黑菊醇A（2）大大增加了C6胶质瘤细胞中神经生长因子（NGF）的产生，其次是corallocin A（4）和hericerin（1）。）。这些化合物增加的NGF产生促进了N2a神经元细胞中的神经突生长。蛋白质印迹分析还显示C6-N2a细胞中NGF，脑源性神经营养因子（BDNF）和突触素（SYP）的蛋白表达增加。综上所述，我们目前的研究表征了猴头肌的神经营养成分，这可能支持记忆改善的潜在用途。