Sclerotinia sclerotiorum (Lib.) de Bary is one of the most dangerous pathogens of rapeseed (Brassica napus L.). Rape plants are infested most often in spring during the flowering phase. The aggressive nature of this fungus as well as its pathogenicity is attributed to its mycotoxin, particularly oxalic acid. Oxalic acid is responsible for the development of acid environment in the plant which triggers off activity of hydrolytic enzymes. The developing crystals of calcium oxalate contribute to destabilisation of pectin compounds found in the cell wall. Moreover, oxalic acid also acts as a suppressor of reactive oxygen forms (H2O2). In this study isolates of the S. sclerotiorum were collected in the years 2012–2014 from three different locations. Collective of isolates described as populations. Two hundred twenty eight (228) isolates of S. sclerotiorum were characterized by evaluation of the total amount of acids produced by the pathogen and at molecular level using Random Amplified Polymorphic DNA (RAPD). Statistical differences were found in acids production by particular S. sclerotiorum isolates collected in the years 2012–2014. Moreover, a relationship was found between acids production and the rate of linear mycelium growth. High similarity between pathogen isolates from the same locality was found using RAPD analysis with twelve primers and small genetic differences between pathogen isolates depending on the place of origin.

中文翻译：

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来自波兰西部的核盘菌 (Lib.) de Bary 分离株的多样性

Sclerotinia sclerotiorum (Lib.) de Bary 是油菜 (Brassica napus L.) 最危险的病原体之一。油菜植物在开花期的春季最常受侵染。这种真菌的侵袭性及其致病性归因于其霉菌毒素，尤其是草酸。草酸负责在植物中形成酸性环境，从而触发水解酶的活性。草酸钙晶体的发育会导致细胞壁中果胶化合物的不稳定。此外，草酸还可以作为活性氧形式 (H2O2) 的抑制剂。在这项研究中，核盘菌的分离株是在 2012-2014 年间从三个不同地点收集的。被描述为种群的分离株的集合。两百二十八 (228) 株 S. 通过使用随机扩增多态性 DNA (RAPD) 在分子水平上评估病原体产生的酸总量来表征核盘菌。在 2012-2014 年收集的特定核盘菌分离物的酸产量中发现了统计差异。此外，还发现了酸产生与线性菌丝体生长速率之间的关系。使用带有十二个引物的 RAPD 分析发现来自同一地点的病原体分离物之间的高度相似性和病原体分离物之间的小的遗传差异取决于起源地。2012-2014 年收集的核盘菌分离物。此外，还发现了酸产生与线性菌丝体生长速率之间的关系。使用带有十二个引物的 RAPD 分析发现来自同一地点的病原体分离物之间的高度相似性和病原体分离物之间的小的遗传差异取决于起源地。2012-2014 年收集的核盘菌分离物。此外，还发现了酸产生与线性菌丝体生长速率之间的关系。使用带有十二个引物的 RAPD 分析发现来自同一地点的病原体分离物之间的高度相似性和病原体分离物之间的小的遗传差异取决于起源地。