Phospholipase C (PLC) responsible for acyl editing of phospholipids plays crucial roles in chilling sensitive plants and flesh fruit, which is prone to physiological disorders caused by chilling stress, collectively termed chilling injury. Up to now, genes encoding PLC in Prunus persica genome (PpPLC) and characteristics closely related to chilling injury remain elusive. In this study, a total of 10 PpPLCs were identified and divided into two subclasses, with equal number of phosphatidylinositol-specific PLC (PpPI-PLCs) and non-specific PLC (PpNPCs). Compared with PpNPCs, PpPI-PLCs show the differences in the number and the organization of exons and introns, as well as multiple splice variants. Meanwhile, the subclasses share two motifs enriched with lysine and proline. Furthermore, the PpPLCs consistently displayed two distinct transcriptional responses to four treatments conferring chilling tolerance in peach fruit, with the upregulation of PpPI-PLCs such as PpPI-PLC4.1/4.2 and the down-regulation of PpNPCs except to PpNPC1, which is located at the plasma membrane. The results will facilitate deciphering the functions of the PLC in plants and flesh fruit when they undergo chilling injury.

负责磷脂的酰基编辑的磷脂酶C（PLC）在冷却敏感植物和果肉中起着至关重要的作用，这种现象容易引起由低温胁迫引起的生理失调，统称为低温伤害。截至目前，在编码基因PLC桃基因组（PpPLC）和密切相关的冷害特征仍然是难以捉摸的。在这项研究中，总共鉴定出10个PpPLC，并将其分为两个亚类，分别具有相同数量的磷脂酰肌醇特异性PLC（PpPI - PLC）和非特异性PLC（PpNPC）。与PpNPC相比，PpPI - PLC显示外显子和内含子在数量和组织上的差异，以及多种剪接变体。同时，这些亚类共有两个富含赖氨酸和脯氨酸的基序。此外，PpPLCs始终显示两个不同的转录反应到四个处理赋予在桃果实耐冷性，具有上调PPPI -的PLC如PPPI - PLC4.1 / 4.2和下调PpNPCs除了PpNPC1，它位于在质膜上 结果将有助于破译植物和果肉在遭受冷害时PLC的功能。