Simultaneous application of polarization microscopy and Interphako interference contrast has been used to study the internal structure of algal cells. The interference contrast technique showed fine cell structures (important is the selection of interference colors according to the Mach-Zehnder interferometer setting). In a polarization microscope, the crossed polarization filters together with the first-order quartz compensator mounted turntable showed the maximum birefringence of the individual structures. Material containing green algae was collected in the villages Sýkořice and Zbečno, Protected Landscape Area (PLA) Křivoklátsko. The objects were studied in a Carl Zeiss Jena NfpK laboratory microscope equipped with an In 160 base body with an Interphako In contrast interference module including a Mach-Zehnder interferometer with variable phase contrast, a special condenser with interchangeable aperture plates, a turntable, a Meopta Praha polarizer, a LOMO Sankt Petersburg analyzer, and a quartz compensator with first-order red and the digital camera DSLR Nikon D 70. Green algae of three orders were studied: Siphonocladales, Zygnematales, and Desmidiales. Anisotropic structures were found in all studied representatives of the green algae of the phylum Chlorophyta. Especially their cell walls showed strong birefringence (in all representatives of these orders). On the other hand, a representative of the order Siphonocladales (the genus Cladophora, Cladophoraceae, Ulvophyceae) was rarely found to display weak birefringent granules of storage substances due to the setting of the Mach-Zehnder interferometer and the use of the first-order compensator (interference colors are intensified). In addition, a very weak birefringence of periphyton cells (microbial biofilm) was found. In the study of the second algae of the genus Spirogyra (Zygnemataceae, Zygnematales, Conjugatophyceae), a strongly birefringent connecting wall between algal cells was found in contrast to the weaker birefringence of the peripheral wall. It was the use of Interphako interference contrast together with polarization filters and a first-order quartz compensator that particularly emphasized the central part of the connecting wall. In the study of the twinned Pleurotaenium algae (Desmidiaceae, Desmidiales, Conjugatophyceae), a strongly birefringent wall was found along the periphery of the cell with a nucleus in the middle part (isthmus). In this narrowing in the center of the cell, a sharply delimited birefringent edge of the cell wall is visible, especially when using Interphako interference contrast along with crossed polarization filters and a first-order quartz compensator. In conclusion, Interphako interference contrast provides a high degree of image contrast in a microscope and, if suitably simultaneously complemented by polarization microscopy (including a first-order quartz compensator), it will allow us to infer some of the composition of the investigated structures. However, working with Interphako interference contrast is considerably more difficult (setting Mach-Zehnder interferometer) than using other contrast techniques (positive and negative phase contrast, color contrast, relief contrast, and dark field).

偏光显微镜和 Interphako 干涉对比的同时应用已被用于研究藻类细胞的内部结构。干涉对比技术显示出精细的细胞结构（重要的是根据 Mach-Zehnder 干涉仪设置选择干涉色）。在偏光显微镜中，交叉偏振滤光片与一阶石英补偿器安装的转台一起显示出单个结构的最大双折射。在风景保护区 (PLA) Křivoklátsko 的 Sýkořice 和 Zbečno 村庄收集了含有绿藻的材料。这些物体是在 Carl Zeiss Jena NfpK 实验室显微镜上进行研究的Praha 偏振器、LOMO Sankt Petersburg 分析仪和具有一阶红色的石英补偿器和数码相机 DSLR Nikon D 70。研究了三阶绿藻：Siphonocladales、Zygnematales 和 Desmidiales。在所有研究的叶绿藻门绿藻代表中都发现了各向异性结构。特别是它们的细胞壁显示出强烈的双折射（在这些订单的所有代表中）。另一方面，虹吸虫目（属 带有可互换孔径板的特殊聚光镜、转盘、Meopta Praha 偏振器、LOMO Sankt Petersburg 分析仪和一阶红色石英补偿器和数码相机 DSLR Nikon D 70。研究了三阶绿藻：Siphonocladales、 Zygnematales 和 Desmidiales。在所有研究的叶绿藻门绿藻代表中都发现了各向异性结构。特别是它们的细胞壁显示出强烈的双折射（在这些订单的所有代表中）。另一方面，虹吸目目（属 带有可互换孔径板的特殊聚光镜、转盘、Meopta Praha 偏振器、LOMO Sankt Petersburg 分析仪和一阶红色石英补偿器和数码相机 DSLR Nikon D 70。研究了三阶绿藻：Siphonocladales、 Zygnematales 和 Desmidiales。在所有研究的叶绿藻门绿藻代表中都发现了各向异性结构。特别是它们的细胞壁显示出强烈的双折射（在这些订单的所有代表中）。另一方面，虹吸虫目（属 和苔藓虫目。在所有研究的叶绿藻门绿藻代表中都发现了各向异性结构。特别是它们的细胞壁显示出强烈的双折射（在这些订单的所有代表中）。另一方面，虹吸虫目（属 和苔藓虫目。在所有研究的叶绿藻门绿藻代表中都发现了各向异性结构。特别是它们的细胞壁显示出强烈的双折射（在这些订单的所有代表中）。另一方面，虹吸虫目（属由于 Mach-Zehnder 干涉仪的设置和一阶补偿器的使用（增强了干涉色），很少发现Cladophora、Cladophoraceae、Ulvophyceae）显示存储物质的弱双折射颗粒。此外，还发现附生生物细胞（微生物生物膜）的双折射非常弱。在对水绵属（Zygnemataceae、Zygnematales、Conjugatophyceae）的第二个藻类的研究中，发现藻类细胞之间存在强双折射的连接壁，而外周壁的双折射较弱。Interphako 干涉对比与偏振滤光片和一阶石英补偿器一起使用，特别强调了连接壁的中心部分。在双胞胎的研究中侧耳草藻类（Desmidiaceae、Desmidiales、Conjugatophyceae），在细胞的外围发现了一个强烈的双折射壁，在中间部分（峡部）有一个细胞核。在细胞中心的这种变窄处，可以看到细胞壁的清晰界定的双折射边缘，尤其是在使用 Interphako 干涉对比以及交叉偏振滤光片和一阶石英补偿器时。总之，Interphako 干涉对比在显微镜中提供了高度的图像对比度，如果同时适当地辅以偏光显微镜（包括一阶石英补偿器），它将使我们能够推断出所研究结构的一些组成。然而，