We studied the effect of zoledronic acid encapsulated into liposomes (L-ZOL) on tumorassociated macrophages in the stroma of hepatocellular carcinoma xenograft. Liposomes were prepared from 1,2-dipalmitoyl-sn-glycero-3-phosphocholine, 1,2-dipalmitoyl-snglycero-3-phospho-sn-1-glycerol, and 1,2-distearoyl-sn-glycero-3-phosphoethanolamine-N-[carboxy(polyethylene glycol)-2000] using thin film method and loaded with zoledronic acid. It was shown that L-ZOL promoted apoptosis of RAW264.7 cells, eliminate much more protumoral M2 macrophages than antitumoral M1 macrophages in the tumor xenograft, and did not significantly reduce the size of xenograft in 6 days. Thus, the effect of treatment depends on the ratio between antitumoral M1 and protumoral M2 polarized macrophages in the tumor.

中文翻译：

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脂质体包裹的唑来膦酸对肝细胞癌微环境的影响可能取决于M1和M2极化巨噬细胞的比例

我们研究了包裹在脂质体中的唑来膦酸 (L-ZOL) 对肝细胞癌异种移植物基质中肿瘤相关巨噬细胞的影响。脂质体由 1,2-二棕榈酰-sn-甘油-3-磷酸胆碱、1,2-二棕榈酰-snglycero-3-磷酸-sn-1-甘油和 1,2-二硬脂酰-sn-甘油-3-磷酸乙醇胺制备-N-[羧基（聚乙二醇）-2000]使用薄膜法并负载唑来膦酸。结果表明，L-ZOL 促进 RAW264.7 细胞凋亡，在肿瘤异种移植物中消除比抗肿瘤 M1 巨噬细胞多得多的原肿瘤 M2 巨噬细胞，并且在 6 天内没有显着减小异种移植物的大小。因此，治疗效果取决于肿瘤中抗肿瘤 M1 和原肿瘤 M2 极化巨噬细胞之间的比率。