To determine the effects of repeated physical activity on iron and zinc homeostases in a living system, we quantified blood and tissue levels of these two metals in sedentary and physically active Long-Evans rats. At post-natal day (PND) 22, female rats were assigned to either a sedentary or an active treatment group (n = 10/group). The physically active rats increased their use of a commercially-constructed stainless steel wire wheel so that, by the end of the study (PND 101), they were running an average of 512.8 ± 31.9 (mean ± standard error) min/night. After euthanization, plasma and aliquots of liver, lung, heart, and gastrocnemius muscle were obtained. Following digestion, non-heme iron and zinc concentrations in plasma and tissues were measured using inductively coupled plasma optical emission spectroscopy. Concentrations of both non-heme iron and zinc in plasma and liver were significantly decreased among the physically active rats relative to the sedentary animals. In the lung, both metals were increased in concentration among the physically active animals but the change in zinc did not reach significance. Similarly, tissue non-heme iron and zinc levels were both increased in heart and muscle from the physically active group. It is concluded that repeated physical activity in an animal model can be associated with a translocation of both iron and zinc from sites of storage (e.g. liver) to tissues with increased metabolism (e.g. the lung, heart, and skeletal muscle).

为了确定重复的身体活动对生命系统中铁和锌稳态的影响，我们量化了久坐和身体活跃的 Long-Evans 大鼠中这两种金属的血液和组织水平。在产后第 22 天 (PND) 时，雌性大鼠被分配到久坐或积极治疗组 (n = 10/组)。身体活跃的大鼠增加了对商业制造的不锈钢线轮的使用，因此，在研究结束时 (PND 101)，它们的平均跑步时间为 512.8 ± 31.9 (平均值 ± 标准误差) 分钟/晚。安乐死后，获得血浆和肝脏、肺、心脏和腓肠肌的等分试样。消化后，使用电感耦合等离子体发射光谱法测量血浆和组织中的非血红素铁和锌浓度。相对于久坐的动物，体力活动的大鼠血浆和肝脏中非血红素铁和锌的浓度显着降低。在肺中，两种金属在体力活动动物中的浓度都增加了，但锌的变化没有达到显着性。同样，运动组的心脏和肌肉组织中的非血红素铁和锌水平均有所增加。得出的结论是，动物模型中重复的身体活动可能与铁和锌从储存部位（例如肝脏）转移到新陈代谢增加的组织（例如肺、心脏和骨骼肌）有关。两种金属在体力活动动物中的浓度都增加了，但锌的变化没有达到显着性。同样，运动组的心脏和肌肉组织中的非血红素铁和锌水平均有所增加。得出的结论是，动物模型中重复的身体活动可能与铁和锌从储存部位（例如肝脏）转移到新陈代谢增加的组织（例如肺、心脏和骨骼肌）有关。两种金属在体力活动动物中的浓度都增加了，但锌的变化没有达到显着性。同样，运动组的心脏和肌肉组织中的非血红素铁和锌水平均有所增加。得出的结论是，动物模型中重复的身体活动可能与铁和锌从储存部位（例如肝脏）转移到新陈代谢增加的组织（例如肺、心脏和骨骼肌）有关。G。肝脏）到新陈代谢增加的组织（例如肺、心脏和骨骼肌）。G。肝脏）到新陈代谢增加的组织（例如肺、心脏和骨骼肌）。