Root-knot nematodes are among the world’s most damaging endoparasitic sedentary nematodes, especially, Meloidogyne incognita that infects a wide range of plant hosts. The activity of different antagonistic bacteria was studied for the low-cost and eco-friendly management of M. incognita on eggplant. Twenty-five isolates were isolated from rhizosphere soils infected with nematodes. Of these, 6 isolates displayed the highest activity, demonstrating 100% mortality of J2 nematodes under laboratory conditions. Partial sequencing of 16S rRNA gene and phylogenetic analysis was used to identify the selected isolates and they were found to be Paenibacillus amylolyticus, Brevibacillus agri, Gluconobacter frateurii, Beijerinckia mobilis, Achromobacter aloeverae, and Pseudomonas stutzeri. The abilities of the selected isolates to produce hydrogen cyanide, siderophores, chitinase, protease, indole acetic acid, and to dissolve phosphorus were also detected. The results of the greenhouse experiment indicated that all the tested bacteria had a greatly significant effectiveness for suppressing root-knot nematode M. incognita. Application of all the rhizosphere bacteria and their combinations reduced the number of galls, number of juveniles, egg-masses, eggs, females, and total final population. All bio-agent treatments succeeded in improving the plant growth parameters and increased the microbial density in eggplant rhizosphere.

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某些根际细菌对根结线虫的影响

根结线虫是世界上最具破坏性的内寄生久坐线虫，尤其是感染广泛植物宿主的根结线虫（Meloidogyne incognita）。研究了不同拮抗细菌的活性，以对茄子上的隐身梭菌进行低成本和生态友好的管理。从感染了线虫的根际土壤中分离出二十五个分离株。其中，有6个分离株表现出最高的活性，表明在实验室条件下J2线虫的死亡率为100％。使用16S rRNA基因的部分测序和系统发育分析来鉴定所选的分离株，发现它们是解淀粉芽孢杆菌，农业短杆菌，费氏葡糖杆菌，运动发酵单胞菌，高产无色杆菌和斯氏假单胞菌。所选菌株产生氰化氢的能力，还检测到铁载体，几丁质酶，蛋白酶，吲哚乙酸和溶解磷。温室实验的结果表明，所有测试的细菌对抑制根结线虫的隐孢子虫都有非常显着的效果。所有根际细菌及其组合的施用减少了gall虫的数量，少年的数量，卵的数量，卵的数量，雌性的数量以及总的最终种群数量。所有生物制剂处理均成功改善了植物的生长参数，并增加了茄子根际的微生物密度。所有根际细菌及其组合的施用减少了s虫的数量，少年的数量，卵的数量，卵的数量，雌性的数量以及总的最终种群数量。所有生物制剂处理均成功改善了植物的生长参数，并增加了茄子根际的微生物密度。所有根际细菌及其组合的施用减少了gall虫的数量，少年的数量，卵的数量，卵的数量，雌性的数量以及总的最终种群数量。所有生物制剂处理均成功改善了植物的生长参数，并增加了茄子根际的微生物密度。