Many medical devices contain metals that interface with the body. Additionally, embedded metal fragments from military wounds are typically not removed, to avoid the risk of morbidity associated with invasive surgery. The long-term health consequences of many of these materials are not thoroughly understood. To this end, we have exposed rats for up to one year to implanted single-element metal pellets of any one of Al, Co, Cu, Fe, Ni, Pb, Ta, or W. Various tissues were harvested and flash frozen for analysis of their metal distribution. We discuss approaches to most thoroughly and reliably evaluate the distribution of metal in these tissues. The path to the most appropriate analytical technique took us through extensive examination of the tissues using Scanning Electron Microscopy with Energy Dispersive X-ray Spectroscopy (SEM-EDS), X-ray Photoelectron Spectroscopy (XPS), and Laser Ablation Inductively Coupled Plamsa Mass Spectrometry (LA-ICP-MS). Though any one of these methods is highly relied upon in surface chemistry analysis, LA-ICP-MS alone showed presence of metal in the tissue. This information will help build robust methods to bridge the gap in our understanding of biosolubility and distribution of embedded metal throughout the body.

中文翻译：

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EXPRESS：用于评估组织中嵌入金属成分的光谱和光谱方法

许多医疗设备都含有与身体接触的金属。此外，军事伤口中嵌入的金属碎片通常不会被移除，以避免与侵入性手术相关的发病风险。许多这些材料的长期健康后果尚未完全了解。为此，我们将大鼠暴露于植入的 Al、Co、Cu、Fe、Ni、Pb、Ta 或 W 中的任何一种的单元素金属丸中长达一年。收集各种组织并进行速冻以进行分析他们的金属分布。我们讨论了最彻底、最可靠地评估金属在这些组织中的分布的方法。通过使用扫描电子显微镜和能量色散 X 射线光谱 (SEM-EDS) 对组织进行广泛检查，我们找到了最合适的分析技术的途径，X 射线光电子能谱 (XPS) 和激光烧蚀电感耦合等离子体质谱 (LA-ICP-MS)。尽管在表面化学分析中高度依赖这些方法中的任何一种，但仅 LA-ICP-MS 就表明组织中存在金属。这些信息将有助于建立可靠的方法，以弥合我们对体内嵌入金属的生物溶解度和分布的理解的差距。