To test the dynamic changes of the expression of genes and microRNA in the gastrocnemius muscle after LPS challenge, 36 piglets were assigned to a control group (slaughtered 0 h after saline injection) and LPS groups (slaughtered at 1 h, 2 h, 4 h, 8 h, and 12 h after LPS treatment, respectively). After LPS treatment, the mRNA expression of IL-1β, IL-6, and TNF-α reached maximal levels at 1 h, 2 h, and 1 h, respectively (P < 0.05), and mRNA expression of TLR4, NODs, muscle-specific ring finger 1, and muscle atrophy F-box peaked at 12 h (P < 0.05). Moreover, the expression of miR-122, miR-135a, and miR-370 reduced at 1 h, 1 h, and 2 h, respectively (P < 0.05), and miR-34a, miR-224, miR-132, and miR-145 reached maximum expression levels at 1 h, 1 h, 2 h, and 4 h, respectively (P < 0.05). These results suggested that mRNA expression of pro-inflammatory cytokines was elevated in the early stage, mRNA expression of genes related to TLR4 and NODs signaling pathways and protein degradation increased in the later phase, and the expression of microRNA related to muscle inflammation and protein degradation changed in the early stage after LPS injection.

为了测试 LPS 攻击后腓肠肌中基因和 microRNA 表达的动态变化，将 36 头仔猪分配到对照组（盐水注射后 0 小时屠宰）和 LPS 组（1 小时、2 小时、4 小时屠宰）。 ，分别在 LPS 处理后 8 小时和 12 小时）。LPS处理后，IL-1β、IL-6、TNF-α的mRNA表达分别在1 h、2 h、1 h达到最大值（P  < 0.05），TLR4、NODs、肌肉的mRNA表达-特异性无名指1，肌肉萎缩F-box在12 h达到峰值（P  < 0.05）。此外，miR-122、miR-135a 和 miR-370 的表达分别在 1 h、1 h 和 2 h 降低（P < 0.05），miR-34a、miR-224、miR-132和miR-145分别在1 h、1 h、2 h和4 h达到最大表达水平（P  < 0.05）。这些结果表明，早期促炎细胞因子mRNA表达升高，后期TLR4和NODs信号通路及蛋白降解相关基因mRNA表达升高，肌肉炎症和蛋白降解相关microRNA表达升高。 LPS 注射后早期发生变化。