Myogenesis is an important and complicated biological process, especially during the process of embryonic development. The homeoprotein Msx1 is a crucial transcriptional repressor of myogenesis and maintains myogenic precursor cells in an undifferentiated, proliferative state. However, the molecular mechanism through which Msx1 coordinates myogenesis remains to be elucidated. Here, we determine the interacting partner proteins of Msx1 in myoblast cells by a proteomic screening method. Msx1 is found to interact with 55 proteins, among which our data demonstrate that the cooperation of Runt-related transcription factor 1 (Runx1) with Msx1 is required for myoblast cell differentiation. Our findings provide important insights into the mechanistic roles of Msx1 in myoblast cell differentiation, and lays foundation for the myogenic differentiation process.

肌生成是一个重要而复杂的生物学过程，尤其是在胚胎发育过程中。同源蛋白 Msx1 是肌生成的关键转录抑制因子，并将肌源性前体细胞维持在未分化的增殖状态。然而，Msx1 协调肌生成的分子机制仍有待阐明。在这里，我们通过蛋白质组学筛选方法确定了成肌细胞中 Msx1 的相互作用伙伴蛋白。发现 Msx1 与 55 种蛋白质相互作用，其中我们的数据表明 Runt 相关转录因子 1 (Runx1) 与 Msx1 的合作是成肌细胞分化所必需的。我们的发现为了解 Msx1 在成肌细胞分化中的机制作用提供了重要见解，